ArticleBMC genomics2010
EcoTILLING in Capsicum species: searching for new virus resistances.
Article in BMC genomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 77 citations in OpenAlex.
- Imprecise Cas12a/ssODN-Mediated Editing of eIF4E1 Confers Dominant-Negative Resistance to Potato Virus Y in Solanum tuberosum.Molecular plant pathology · 2026Article
- Molecular gatekeepers: eukaryotic translation factors decoding plant-virus dynamics for resistance engineering.Stress biology · 2026Review
- Molecular and genomic insights into viral resistance inFrontiers in plant science · 2025Review
- The Naturally Occurring Amino Acid Substitution in the VPg α1-α2 Loop Breaks eIF4E-Mediated Resistance to PRSV by Enabling VPg to Re-Hijack Another eIF4E Isoform eIF(iso)4E in Watermelon.Molecular plant pathology · 2024Article
- Current knowledge and breeding strategies for management of aphid-transmitted viruses of pepper (Frontiers in plant science · 2024Review
- Allele mining of eukaryotic translation initiation factor genes in Prunus for the identification of new sources of resistance to sharka.Scientific reports · 2023Article
- Plant eIF4E isoforms as factors of susceptibility and resistance to potyviruses.Frontiers in plant science · 2023Review
- Translation Initiation Factor eIF4E Positively Modulates Conidiogenesis, Appressorium Formation, Host Invasion and Stress Homeostasis in the Filamentous FungiFrontiers in plant science · 2021Article
- Translational gene regulation in plants: A green new deal.Wiley interdisciplinary reviews. RNA · 2020Review
- Plant Genome Editing and the Relevance of Off-Target Changes.Plant physiology · 2020Article
- Overview of Biotic Stresses in Pepper (International journal of molecular sciences · 2020Review
- A mutation in the melon Vacuolar Protein Sorting 41prevents systemic infection of Cucumber mosaic virus.Scientific reports · 2017Article
- Sequence diversification in recessive alleles of two host factor genes suggests adaptive selection for bymovirus resistance in cultivated barley from East Asia.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2017Article
- Deficiency of the eIF4E isoform nCBP limits the cell-to-cell movement of a plant virus encoding triple-gene-block proteins in Arabidopsis thaliana.Scientific reports · 2017Article
- Human Management of a Wild Plant Modulates the Evolutionary Dynamics of a Gene Determining Recessive Resistance to Virus Infection.PLoS genetics · 2016Article
- EcoTILLING-Based Association Mapping Efficiently Delineates Functionally Relevant Natural Allelic Variants of Candidate Genes Governing Agronomic Traits in Chickpea.Frontiers in plant science · 2016Article
- Tomato Fruits Show Wide Phenomic Diversity but Fruit Developmental Genes Show Low Genomic Diversity.PloS one · 2016Article
- Plant Translation Factors and Virus Resistance.Viruses · 2015Review
- State of the science and challenges of breeding landscape plants with ecological function.Horticulture research · 2015Review
- Transgenic Brassica rapa plants over-expressing eIF(iso)4E variants show broad-spectrum Turnip mosaic virus (TuMV) resistance.Molecular plant pathology · 2014Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe EcoTILLING technique allows polymorphisms in target genes of natural populations to be quickly analysed or identified and facilitates the screening of genebank collections for desired traits. We have developed an EcoTILLING platform to exploit Capsicum genetic resources. A perfect example of the utility of this EcoTILLING platform is its application in searching for new virus-resistant alleles in Capsicum genus. Mutations in translation initiation factors (eIF4E, eIF(iso)4E, eIF4G and eIF(iso)4G) break the cycle of several RNA viruses without affecting the plant life cycle, which makes these genes potential targets to screen for resistant germplasm.
resultsWe developed and assayed a cDNA-based EcoTILLING platform with 233 cultivated accessions of the genus Capsicum. High variability in the coding sequences of the eIF4E and eIF(iso)4E genes was detected using the cDNA platform. After sequencing, 36 nucleotide changes were detected in the CDS of eIF4E and 26 in eIF(iso)4E. A total of 21 eIF4E haplotypes and 15 eIF(iso)4E haplotypes were identified. To evaluate the functional relevance of this variability, 31 possible eIF4E/eIF(iso)4E combinations were tested against Potato virus Y. The results showed that five new eIF4E variants (pvr2(10), pvr2(11), pvr2(12), pvr2(13) and pvr2(14)) were related to PVY-resistance responses.
conclusionsEcoTILLING was optimised in different Capsicum species to detect allelic variants of target genes. This work is the first to use cDNA instead of genomic DNA in EcoTILLING. This approach avoids intronic sequence problems and reduces the number of reactions. A high level of polymorphism has been identified for initiation factors, showing the high genetic variability present in our collection and its potential use for other traits, such as genes related to biotic or abiotic stresses, quality or production. Moreover, the new eIF4E and eIF(iso)4E alleles are an excellent collection for searching for new resistance against other RNA viruses.
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