ArticleJournal of biomedical science2010
Prenatal exposure of ethanol induces increased glutamatergic neuronal differentiation of neural progenitor cells.
Article in Journal of biomedical science, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- Article
- Decreased Cerebral Blood Flow in Young Children With Prenatal Alcohol Exposure.Biological psychiatry global open science · 2025Article
- Effects of alcohol on the transcriptome, methylome and metabolome of in vitro gastrulating human embryonic cells.Disease models & mechanisms · 2025Article
- Upregulated GIRK2 Counteracts Ethanol-Induced Changes in Excitability and Respiration in Human Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Prenatal alcohol exposure promotes NLRP3 inflammasome-dependent immune actions following morphine treatment and paradoxically prolongs nerve injury-induced pathological pain in female mice.Alcohol, clinical & experimental research · 2023Article
- Binge-like Prenatal Ethanol Exposure Causes Impaired Cellular Differentiation in the Embryonic Forebrain and Synaptic and Behavioral Defects in Adult Mice.Brain sciences · 2022Article
- Cell type-specific changes in Wnt signaling and neuronal differentiation in the developing mouse cortex after prenatal alcohol exposure during neurogenesis.Frontiers in cell and developmental biology · 2022Article
- Prenatal Exposure to Alcohol Induces Functional and Structural Plasticity in Dopamine D1 Receptor-Expressing Neurons of the Dorsomedial Striatum.Alcoholism, clinical and experimental research · 2018Article
- Chromatin Switches during Neural Cell Differentiation and Their Dysregulation by Prenatal Alcohol Exposure.Genes · 2017Review
- Chronic alcohol exposure affects the cell components involved in membrane traffic in neuronal dendrites.Neurotoxicity research · 2015Article
- Stem cell therapy: a new approach to the treatment of refractory depression.Journal of neural transmission (Vienna, Austria : 1996) · 2014Article
- Prenatal alcohol exposure alters expression of neurogenesis-related genes in an ex vivo cell culture model.Alcohol (Fayetteville, N.Y.) · 2014Article
- Pax6-dependent cortical glutamatergic neuronal differentiation regulates autism-like behavior in prenatally valproic acid-exposed rat offspring.Molecular neurobiology · 2014Article
- Chronic alcohol alters dendritic spine development in neurons in primary culture.Neurotoxicity research · 2013Article
- Prenatal alcohol exposure and cellular differentiation: a role for Polycomb and Trithorax group proteins in FAS phenotypes?Alcohol research : current reviews · 2013Review
- Stem cell therapy: social recognition recovery in a FASD model.Translational psychiatry · 2012Article
- Developmental neurotoxicity screening using human embryonic stem cells.Experimental neurology · 2012Article
- Developmental changes in embryonic hypothalamic neurons during prenatal fat exposure.American journal of physiology. Endocrinology and metabolism · 2012Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPrenatal ethanol exposure during pregnancy induces a spectrum of mental and physical disorders called fetal alcohol spectrum disorder (FASD). The central nervous system is the main organ influenced by FASD, and neurological symptoms include mental retardation, learning abnormalities, hyperactivity and seizure susceptibility in childhood along with the microcephaly. In this study, we examined whether ethanol exposure adversely affects the proliferation of NPC and de-regulates the normal ratio between glutamatergic and GABAergic neuronal differentiation using primary neural progenitor culture (NPC) and in vivo FASD models.
methodsNeural progenitor cells were cultured from E14 embryo brain of Sprague-Dawley rat. Pregnant mice and rats were treated with ethanol (2 or 4 g/kg/day) diluted with normal saline from E7 to E16 for in vivo FASD animal models. Expression level of proteins was investigated by western blot analysis and immunocytochemical assays. MTT was used for cell viability. Proliferative activity of NPCs was identified by BrdU incorporation, immunocytochemistry and FACS analysis.
resultsReduced proliferation of NPCs by ethanol was demonstrated using BrdU incorporation, immunocytochemistry and FACS analysis. In addition, ethanol induced the imbalance between glutamatergic and GABAergic neuronal differentiation via transient increase in the expression of Pax6, Ngn2 and NeuroD with concomitant decrease in the expression of Mash1. Similar pattern of expression of those transcription factors was observed using an in vivo model of FASD as well as the increased expression of PSD-95 and decreased expression of GAD67.
conclusionsThese results suggest that ethanol induces hyper-differentiation of glutamatergic neuron through Pax6 pathway, which may underlie the hyper-excitability phenotype such as hyperactivity or seizure susceptibility in FASD patients.
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