ArticleThe European journal of neuroscience2010
Quantitative localisation of synaptic and extrasynaptic GABAA receptor subunits on hippocampal pyramidal cells by freeze-fracture replica immunolabelling.
Article in The European journal of neuroscience, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers.
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91 citing papers in PubMed, 147 citations in OpenAlex.
- Spatial Deconstruction of the Plasma Membrane.Journal of the American Chemical Society · 2026Article
- The role of GABANeuroscience and biobehavioral reviews · 2026Review
- Distinct nanoscale architectures of GABAergic inhibitory synapses predict diverse synaptic output.bioRxiv : the preprint server for biology · 2026Article
- Decoding the structural and functional diversity of GABAFrontiers in pharmacology · 2025Review
- The GABANeuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2023Article
- Layer-specific distribution and expression pattern of AMPA- and NMDA-type glutamate receptors in the barrel field of the adult rat somatosensory cortex: a quantitative electron microscopic analysis.Cerebral cortex (New York, N.Y. : 1991) · 2023Article
- Therapeutic effects of KRM-II-81, positive allosteric modulator for α2/3 subunit containing GABAFrontiers in pharmacology · 2023Article
- Recruitment of Plasma Membrane GABA-A Receptors by Submembranous Gephyrin/Collybistin Clusters.Cellular and molecular neurobiology · 2022Article
- FMRP regulates GABACell reports · 2022Article
- Cellular and molecular signatures of in vivo imaging measures of GABAergic neurotransmission in the human brain.Communications biology · 2022Article
- Tonic GABAThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- GABAJournal, genetic engineering & biotechnology · 2021Review
- Mechanisms of GABAergic and cholinergic neurotransmission in auditory thalamus: Impact of aging.Hearing research · 2021Review
- Relocation of an Extrasynaptic GABANeuron · 2021Article
- The Epilepsy-Related Protein PCDH19 Regulates Tonic Inhibition, GABAMolecular neurobiology · 2020Article
- Inhibitory synaptic transmission tuned by CaDevelopment, growth & differentiation · 2020Review
- Article
- Enhancing neuronal chloride extrusion rescues α2/α3 GABANature communications · 2020Article
- Lack of Neurosteroid Selectivity at δ vs. γ2-Containing GABAFrontiers in molecular neuroscience · 2020Article
- Steady-state activation and modulation of the synaptic-type α1β2γ2L GABAPhysiological reports · 2019Article
31 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 5 institutions in 4 countries.
Funding
Abstract
Hippocampal CA1 pyramidal cells, which receive γ-aminobutyric acid (GABA)ergic input from at least 18 types of presynaptic neuron, express 14 subunits of the pentameric GABA(A) receptor. The relative contribution of any subunit to synaptic and extrasynaptic receptors influences the dynamics of GABA and drug actions. Synaptic receptors mediate phasic GABA-evoked conductance and extrasynaptic receptors contribute to a tonic conductance. We used freeze-fracture replica-immunogold labelling, a sensitive quantitative immunocytochemical method, to detect synaptic and extrasynaptic pools of the alpha1, alpha2 and beta3 subunits. Antibodies to the cytoplasmic loop of the subunits showed immunogold particles concentrated on distinct clusters of intramembrane particles (IMPs) on the cytoplasmic face of the plasma membrane on the somata, dendrites and axon initial segments, with an abrupt decrease in labelling at the edge of the IMP cluster. Neuroligin-2, a GABAergic synapse-specific adhesion molecule, co-labels all beta3 subunit-rich IMP clusters, therefore we considered them synapses. Double-labelling for two subunits showed that virtually all somatic synapses contain the alpha1, alpha2 and beta3 subunits. The extrasynaptic plasma membrane of the somata, dendrites and dendritic spines showed low-density immunolabelling. Synaptic labelling densities on somata for the alpha1, alpha2 and beta3 subunits were 78-132, 94 and 79 times higher than on the extrasynaptic membranes, respectively. As GABAergic synapses occupy 0.72% of the soma surface, the fraction of synaptic labelling was 33-48 (alpha1), 40 (alpha2) and 36 (beta3)% of the total somatic surface immunolabelling. Assuming similar antibody access to all receptors, about 60% of these subunits are in extrasynaptic receptors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.