Evidence map›Paper›PMID 21072521›Full record

ArticlePlant cell reports2011

Analysis of target sequences of DDM1s in Brassica rapa by MSAP.

Taku Sasaki, Ryo Fujimoto, Sachie Kishitani, Takeshi Nishio

Abstract read
PubMed Publisher
In one paragraph

Article in Plant cell reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
3.3field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 22 citations in OpenAlex.

  1. Whole-Genome DNA Methylation Analysis inFrontiers in plant science · 2022
    Article
  2. Review
  3. Review
  4. Genome-wide characterization of DNA methylation, small RNA expression, and histone H3 lysine nine di-methylation in Brassica rapa L.DNA research : an international journal for rapid publication of reports on genes and genomes · 2018
    Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Molecular mechanisms of epigenetic variation in plants.International journal of molecular sciences · 2012
    Review
  10. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 3 countries.

Taku SasakiGraduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, Miyagi, 981-8555, Japan.
Ryo Fujimoto
Sachie Kishitani
Takeshi Nishio
Tohoku University · JPAustrian Academy of Sciences · ATPlant Industry · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

DNA methylation is an important epigenetic modification regulating gene expression and transposon silencing. Although epigenetic regulation is involved in some agricultural traits, there has been relatively little research on epigenetic modifications of genes in Brassica rapa, which includes many important vegetables. In B. rapa, orthologs of DDM1, a chromatin remodeling factor required for maintenance of DNA methylation, have been characterized and DNA hypomethylated knock-down plants by RNAi (ddm1-RNAi plants) have been generated. In this study, we investigated differences of DNA methylation status at the genome-wide level between a wild-type (WT) plant and a ddm1-RNAi plant by methylation-sensitive amplification polymorphism (MSAP) analysis. MSAP analysis detected changes of DNA methylation of many repetitive sequences in the ddm1-RNAi plant. Search for body methylated regions in the WT plant revealed no difference in gene body methylation levels between the WT plant and the ddm1-RNAi plant. These results indicate that repetitive sequences are preferentially methylated by DDM1 genes in B. rapa.

Indexed as

Polymorphism, GeneticBase SequenceBlotting, SouthernBrassica rapaDNA MethylationOpen Reading FramesPlant ProteinsPolymerase Chain ReactionRNA InterferenceSequence Analysis, DNAPlant Proteins

Identifiers

PMID21072521
OpenAlexW1984027358

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.