ArticlePLoS neglected tropical diseases2010
Serological evaluation of Mycobacterium ulcerans antigens identified by comparative genomics.
Article in PLoS neglected tropical diseases, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- Cell-mediated and serology-based tests for Mycobacterium ulcerans disease: A systematic review and meta-analysis.PLoS neglected tropical diseases · 2020Pooled it
- Buruli ulcer.Nature reviews. Disease primers · 2025Review
- Spatiotemporal distribution ofEmerging microbes & infections · 2025Article
- A single synthetic lipid antigen for improved serological diagnosis of Buruli ulcer.Public health action · 2023Article
- A human model of Buruli ulcer: The case for controlled human infection and considerations for selecting a Mycobacterium ulcerans challenge strain.PLoS neglected tropical diseases · 2023Review
- Vaccine-Specific Immune Responses against Mycobacterium ulcerans Infection in a Low-Dose Murine Challenge Model.Infection and immunity · 2020Article
- Article
- In Vivo Imaging of BioluminescentThe American journal of tropical medicine and hygiene · 2019Article
- Article
- A Sero-epidemiological Approach to Explore Transmission of Mycobacterium ulcerans.PLoS neglected tropical diseases · 2016Article
- Clinical and Laboratory Diagnosis of Buruli Ulcer Disease: A Systematic Review.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2016Review
- Analysis of the metabolome of Anopheles gambiae mosquito after exposure to Mycobacterium ulcerans.Scientific reports · 2015Article
- Identification of the Mycobacterium ulcerans protein MUL_3720 as a promising target for the development of a diagnostic test for Buruli ulcer.PLoS neglected tropical diseases · 2015Article
- Analysis of the vaccine potential of plasmid DNA encoding nine mycolactone polyketide synthase domains in Mycobacterium ulcerans infected mice.PLoS neglected tropical diseases · 2014Article
- Environmental distribution and seasonal prevalence of Mycobacterium ulcerans in Southern Louisiana.Applied and environmental microbiology · 2013Article
- The cell wall-associated mycolactone polyketide synthases are necessary but not sufficient for mycolactone biosynthesis.PloS one · 2013Article
- Sero-epidemiology as a tool to screen populations for exposure to Mycobacterium ulcerans.PLoS neglected tropical diseases · 2012Article
- Spontaneous clearance of Mycobacterium ulcerans in a case of Buruli ulcer.PLoS neglected tropical diseases · 2011Article
- Treating Mycobacterium ulcerans disease (Buruli ulcer): from surgery to antibiotics, is the pill mightier than the knife?Future microbiology · 2011Review
- Sculpting the immune response to infection.Nature immunology · 2011Article
Corrections and comments
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Authors and funding
14 authors at 8 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A specific and sensitive serodiagnostic test for Mycobacterium ulcerans infection would greatly assist the diagnosis of Buruli ulcer and would also facilitate seroepidemiological surveys. By comparative genomics, we identified 45 potential M. ulcerans specific proteins, of which we were able to express and purify 33 in E. coli. Sera from 30 confirmed Buruli ulcer patients, 24 healthy controls from the same endemic region and 30 healthy controls from a non-endemic region in Benin were screened for antibody responses to these specific proteins by ELISA. Serum IgG responses of Buruli ulcer patients were highly variable, however, seven proteins (MUP045, MUP057, MUL_0513, Hsp65, and the polyketide synthase domains ER, AT propionate, and KR A) showed a significant difference between patient and non-endemic control antibody responses. However, when sera from the healthy control subjects living in the same Buruli ulcer endemic area as the patients were examined, none of the proteins were able to discriminate between these two groups. Nevertheless, six of the seven proteins showed an ability to distinguish people living in an endemic area from those in a non-endemic area with an average sensitivity of 69% and specificity of 88%, suggesting exposure to M. ulcerans. Further validation of these six proteins is now underway to assess their suitability for use in Buruli ulcer seroepidemiological studies. Such studies are urgently needed to assist efforts to uncover environmental reservoirs and understand transmission pathways of the M. ulcerans.
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