ArticleThe Journal of clinical investigation1990
Measurement of very low density and low density lipoprotein apolipoprotein (Apo) B-100 and high density lipoprotein Apo A-I production in human subjects using deuterated leucine. Effect of fasting and feeding.
Article in The Journal of clinical investigation, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 194 citations in OpenAlex.
- Effects of Therapeutic Lifestyle Change diets high and low in dietary fish-derived FAs on lipoprotein metabolism in middle-aged and elderly subjects.Journal of lipid research · 2012Trial
- VLDL lipolysis products increase VLDL fluidity and convert apolipoprotein E4 into a more expanded conformation.Journal of lipid research · 2010Trial
- Effects of cholesteryl ester transfer protein inhibition on apolipoprotein A-II-containing HDL subspecies and apolipoprotein A-II metabolism.Journal of lipid research · 2009Trial
- Effects of ezetimibe and simvastatin on apolipoprotein B metabolism in males with mixed hyperlipidemia.Journal of lipid research · 2009 · on this mapTrial
- Extended-release niacin alters the metabolism of plasma apolipoprotein (Apo) A-I and ApoB-containing lipoproteins.Arteriosclerosis, thrombosis, and vascular biology · 2008Trial
- Role of the estrogen and progestin in hormonal replacement therapy on apolipoprotein A-I kinetics in postmenopausal women.Arteriosclerosis, thrombosis, and vascular biology · 2006Trial
- Intracellular tPA-PAI-1 interaction determines VLDL assembly in hepatocytes.Science (New York, N.Y.) · 2023Article
- Assessing HDL Metabolism in Subjects with Elevated Levels of HDL Cholesterol and Coronary Artery Disease.Molecules (Basel, Switzerland) · 2021Article
- Effect of fasting and feeding on apolipoprotein A-I kinetics in preβScientific reports · 2020Article
- Plasma PCSK9 correlates with apoB-48-containing triglyceride-rich lipoprotein production in men with insulin resistance.Journal of lipid research · 2018Article
- Metabolism and proteomics of large and small dense LDL in combined hyperlipidemia: effects of rosuvastatin.Journal of lipid research · 2017Article
- Role of NCAN rs2228603 polymorphism in the incidence of nonalcoholic fatty liver disease: a case-control study.Lipids in health and disease · 2016Article
- Distinct metabolism of apolipoproteins (a) and B-100 within plasma lipoprotein(a).Metabolism: clinical and experimental · 2016Article
- Practical immunoaffinity-enrichment LC-MS for measuring protein kinetics of low-abundance proteins.Clinical chemistry · 2014Article
- Genetic variation at NCAN locus is associated with inflammation and fibrosis in non-alcoholic fatty liver disease in morbid obesity.Human heredity · 2013Article
- Development of a method to measure preβHDL and αHDL apoA-I enrichment for stable isotopic studies of HDL kinetics.Lipids · 2012Article
- Plasma proteome dynamics: analysis of lipoproteins and acute phase response proteins with 2H2O metabolic labeling.Molecular & cellular proteomics : MCP · 2012Article
- Effects of CETP inhibition on triglyceride-rich lipoprotein composition and apoB-48 metabolism.Journal of lipid research · 2012Article
- Postprandial apoE isoform and conformational changes associated with VLDL lipolysis products modulate monocyte inflammation.PloS one · 2012Article
- Fatty acids from very low-density lipoprotein lipolysis products induce lipid droplet accumulation in human monocytes.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Six normolipidemic male subjects, after an 8-h overnight fast, were given a bolus injection and then a 15-h constant intravenous infusion of [D3]L-leucine. Subjects were studied in the fasted state and on a second occasion in the fed state (small, physiological meals were given every hour for 15 h). Apolipoproteins were isolated by preparative gradient gel electrophoresis from plasma lipoproteins separated by sequential ultracentrifugation. Incorporation of [D3]L-leucine into apolipoproteins was monitored by negative ionization, gas chromatography-mass spectrometry. Production rates were determined by multiplying plasma apolipoprotein pool sizes by fractional production rates (calculated as the rate of isotopic enrichment [IE] of each protein as a fraction of IE achieved by VLDL (d less than 1.006 g/ml) apo B-100 at plateau. VLDL apo B-100 production was greater, and LDL (1.019 less than d less than 1.063 g/ml) apo B-100 production was less in the fed compared with the fasted state (9.9 +/- 1.7 vs. 6.4 +/- 1.7 mg/kg per d, P less than 0.01, and 8.9 +/- 1.2 vs. 13.1 +/- 1.2 mg/kg per d, P less than 0.05, respectively). No mean change was observed in high density lipoprotein apo A-I production. We conclude that: (a) this stable isotope, endogenous-labeling technique, for the first time allows for the in vivo measurement of apolipoprotein production in the fasted and fed state; and (b) since LDL apo B-100 production was greater than VLDL apo B-100 production in the fasted state, this study provides in vivo evidence that LDL apo B-100 can be produced independently of VLDL apo B-100 in normolipidemic subjects.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.