ArticleExperimental & molecular medicine2010
Triptolide downregulates human GD3 synthase (hST8Sia I) gene expression in SK-MEL-2 human melanoma cells.
Article in Experimental & molecular medicine, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- Ganglioside GD2 identifies breast cancer stem cells and promotes tumorigenesis.The Journal of clinical investigation · 2012Trial
- GD3s-mediated lipid metabolism reprograming promotes proliferation and metastasis of melanoma.Journal of translational medicine · 2026Article
- Chloroform Extract from FermentedJournal of microbiology and biotechnology · 2024Article
- Regulatory mechanism for the human glioblastoma cell-specific expression of the human GD1c/GT1a/GQ1b synthase (hST8Sia V) gene.Glycoconjugate journal · 2023Article
- Transcription of human β-galactoside α2,6-sialyltransferase (hST6Gal I) is downregulated by curcumin through AMPK signaling in human colon carcinoma HCT116 cells.Genes & genomics · 2023Article
- Article
- The biological role and immunotherapy of gangliosides and GD3 synthase in cancers.Frontiers in cell and developmental biology · 2023Review
- Role of GD3 Synthase ST8Sia I in Cancers.Cancers · 2022Review
- Curcumin-mediated transcriptional regulation of humanFrontiers in molecular biosciences · 2022Article
- Gangliosides as Signaling Regulators in Cancer.International journal of molecular sciences · 2021Review
- Comprehensive Transcriptomic Analysis IdentifiesGenes · 2020Article
- Upregulation of cell surface GD3 ganglioside phenotype is associated with human melanoma brain metastasis.Molecular oncology · 2020Article
- EMT-related protein expression in polyploid giant cancer cells and their daughter cells with different passages after triptolide treatment.Medical oncology (Northwood, London, England) · 2019Article
- Ganglioside GD3 synthase (GD3S), a novel cancer drug target.Acta pharmaceutica Sinica. B · 2018Review
- Transcriptional Activation of Human GD3 Synthase (hST8Sia I) Gene in Curcumin-Induced Autophagy in A549 Human Lung Carcinoma Cells.International journal of molecular sciences · 2018Article
- Upregulation of Human ST8Sia VI (α2,8-Sialyltransferase) Gene Expression by Physcion in SK-N-BE(2)-C Human Neuroblastoma Cells.International journal of molecular sciences · 2016Article
- Promoters of Human Cosmc and T-synthase Genes Are Similar in Structure, Yet Different in Epigenetic Regulation.The Journal of biological chemistry · 2015Article
- GD3 synthase regulates epithelial-mesenchymal transition and metastasis in breast cancer.Oncogene · 2015Article
- Dysregulated Expression of Glycolipids in Tumor Cells: From Negative Modulator of Anti-tumor Immunity to Promising Targets for Developing Therapeutic Agents.Frontiers in oncology · 2015Review
- GD2 in Breast Cancer: A Potential Biomarker and Therapeutic Target.Cancer genomics & proteomicsReview
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, we have shown that gene expression of human GD3 synthase (hST8Sia I) is suppressed by triptolide (TPL) in human melanoma SK-MEL-2 cells. To elucidate the mechanism underlying the downregulation of hST8Sia I gene expression in TPL-treated SK-MEL-2 cells, we characterized the TPL-inducible promoter region within the hST8Sia I gene using luciferase constructs carrying 5'-deletions of the hST8Sia I promoter. Functional analysis of the 5'-flanking region of the hST8Sia I gene demonstrated that the -1146 to -646 region, which contains putative binding sites for transcription factors c-Ets-1, CREB, AP-1 and NF-κB, functions as the TPL-inducible promoter of hST8Sia I in SK-MEL-2 cells. Site-directed mutagenesis and ChIP analysis indicated that the NF-κB binding site at -731 to -722 is crucial for TPL-induced suppression of hST8Sia I in SK-MEL-2 cells. This suggests that TPL induces down-regulation of hST8Sia I gene expression through NF-κB activation in human melanoma cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.