ArticleProceedings of the National Academy of Sciences of the United States of America2010
Cell-free fusion of bacteria-containing phagosomes with endocytic compartments.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 52 citations in OpenAlex.
- Advancing antifungal therapy: exploring targeted CFW-PEc-enhanced ethosomal formulations of amphotericin B against cryptococcal pneumonia.Microbiology spectrum · 2025Article
- Syntaxin-2 balances phagocytic uptake and phagolysosomal clearance in macrophages.Journal of cell science · 2025Article
- Quantitative modeling of rod outer segment phagocytosis and recycling.Scientific reports · 2025Article
- Combating Tuberculosis via Restoring the Host Immune Capacity by TargetingInternational journal of molecular sciences · 2024Review
- Methods for investigating STAT3 regulation of lysosomal function in mammary epithelial cells.Journal of mammary gland biology and neoplasia · 2024Article
- Consecutive functions of small GTPases guide HOPS-mediated tethering of late endosomes and lysosomes.Cell reports · 2023Article
- Biochemically Reconstituted Fusion of Phagosomes with Endosomes and Lysosomes.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Cell-Level Analysis Visualizing Photodynamic Therapy with Porphylipoprotein and Talaporphyrin Sodium.International journal of molecular sciences · 2022Article
- Kinetics of phagosome maturation is coupled to their intracellular motility.Communications biology · 2022Article
- Single-phagosome imaging reveals that homotypic fusion impairs phagosome degradative function.Biophysical journal · 2022Article
- SNARE Proteins Mediate α-Synuclein Secretion via Multiple Vesicular Pathways.Molecular neurobiology · 2022Article
- Macrophage Proteome Analysis at Different Stages ofProteomes · 2021Article
- A guide to measuring phagosomal dynamics.The FEBS journal · 2021Review
- Rab GTPases in the differential processing of phagocytosed pathogens versus efferocytosed apoptotic cells.Histology and histopathology · 2021Review
- Role of Apoptotic Cell Clearance in Pneumonia and Inflammatory Lung Disease.Pathogens (Basel, Switzerland) · 2021Review
- Better Together: Current Insights Into Phagosome-Lysosome Fusion.Frontiers in immunology · 2021Review
- Rab GTPases take centre stage in understandingSmall GTPases · 2020Review
- Formation and Maturation of the Phagosome: A Key Mechanism in Innate Immunity against Intracellular Bacterial Infection.Microorganisms · 2020Review
- Sialic Acid-Siglec-E Interactions DuringFrontiers in immunology · 2020Article
- Harnessing Macrophages for Controlled-Release Drug Delivery: Lessons From Microbes.Frontiers in pharmacology · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Uptake of microorganisms by professional phagocytic cells leads to formation of a new subcellular compartment, the phagosome, which matures by sequential fusion with early and late endocytic compartments, resulting in oxidative and nonoxidative killing of the enclosed microbe. Few tools are available to study membrane fusion between phagocytic and late endocytic compartments in general and with pathogen-containing phagosomes in particular. We have developed and applied a fluorescence microscopy assay to study fusion of microbe-containing phagosomes with different-aged endocytic compartments in vitro. This revealed that fusion of phagosomes containing nonpathogenic Escherichia coli with lysosomes requires Rab7 and SNARE proteins but not organelle acidification. In vitro fusion experiments with phagosomes containing pathogenic Salmonella enterica serovar Typhimurium indicated that reduced fusion of these phagosomes with early and late endocytic compartments was independent of endosome and cytosol sources and, hence, a consequence of altered phagosome quality.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.