ArticleScience (New York, N.Y.)2010
How the CCA-adding enzyme selects adenine over cytosine at position 76 of tRNA.
Article in Science (New York, N.Y.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Intrinsic catalytic parameters drive specificity of non-templated poly(UG) tail synthesis.Nucleic acids research · 2026Article
- Enzyme structure and kinetics produce tRNA and nucleotide specificity of Schizosaccharomyces pombe CC- and A-adding enzymes.Nucleic acids research · 2026Article
- Crystal structure of the CCA-adding enzyme fromJournal of structural biology: X · 2025Article
- Importance of residue 248 in Escherichia coli RNase P RNA mediated cleavage.Scientific reports · 2023Article
- The life and times of a tRNA.RNA (New York, N.Y.) · 2023Review
- A unified dinucleotide alphabet describing both RNA and DNA structures.Nucleic acids research · 2020Article
- Thomas A. Steitz (1940-2018).RNA (New York, N.Y.) · 2019Article
- Function and Regulation of Human Terminal Uridylyltransferases.Frontiers in genetics · 2018Review
- RPI-Bind: a structure-based method for accurate identification of RNA-protein binding sites.Scientific reports · 2017Article
- Computational investigation of proton transfer, pKa shifts and pH-optimum of protein-DNA and protein-RNA complexes.Proteins · 2017Article
- Mitochondrial poly(A) polymerase is involved in tRNA repair.Nucleic acids research · 2015Article
- Article
- Structures and functions of Qβ replicase: translation factors beyond protein synthesis.International journal of molecular sciences · 2014Review
- Molecular mechanisms of template-independent RNA polymerization by tRNA nucleotidyltransferases.Frontiers in genetics · 2014Review
- How a CCA sequence protects mature tRNAs and tRNA precursors from action of the processing enzyme RNase BN/RNase Z.The Journal of biological chemistry · 2013Article
- An inhibitory C-terminal region dictates the specificity of A-adding enzymes.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- tRNAs marked with CCACCA are targeted for degradation.Science (New York, N.Y.) · 2011Article
- Role of tRNA amino acid-accepting end in aminoacylation and its quality control.Nucleic acids research · 2011Article
- A model of proto-anti-codon RNA enzymes requiring L-amino acid homochirality.Journal of molecular evolution · 2011Article
- Controlling translation via modulation of tRNA levels.Wiley interdisciplinary reviews. RNAReview
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Authors and funding
3 authors.
Funding
Abstract
CCA-adding enzymes [ATP(CTP):tRNA nucleotidyltransferases] add CCA onto the 3' end of transfer RNA (tRNA) precursors without using a nucleic acid template. Although the mechanism by which cytosine (C) is selected at position 75 of tRNA has been established, the mechanism by which adenine (A) is selected at position 76 remains elusive. Here, we report five cocrystal structures of the enzyme complexed with both a tRNA mimic and nucleoside triphosphates under catalytically active conditions. These structures suggest that adenosine 5'-monophosphate is incorporated onto the A76 position of the tRNA via a carboxylate-assisted, one-metal-ion mechanism with aspartate 110 functioning as a general base. The discrimination against incorporation of cytidine 5'-triphosphate (CTP) at position 76 arises from improper placement of the α phosphate of the incoming CTP, which results from the interaction of C with arginine 224 and prevents the nucleophilic attack by the 3' hydroxyl group of cytidine75.
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