ArticleNucleic acids research2011
The ARiBo tag: a reliable tool for affinity purification of RNAs under native conditions.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- Stimulus-responsive assembly of nonviral nucleocapsids.Nature communications · 2024Article
- m6A and YTHDF proteins contribute to the localization of select neuronal mRNAs.Nucleic acids research · 2022Article
- TDP-43 stabilizes G3BP1 mRNA: relevance to amyotrophic lateral sclerosis/frontotemporal dementia.Brain : a journal of neurology · 2021Article
- Tagging and Capturing of Lentiviral Vectors Using Short RNAs.International journal of molecular sciences · 2021Article
- Evolution of a virus-like architecture and packaging mechanism in a repurposed bacterial protein.Science (New York, N.Y.) · 2021Article
- Challenges to optimizing RNA nanostructures for large scale production and controlled therapeutic properties.Nanomedicine (London, England) · 2020Article
- Laboratory evolution of virus-like nucleocapsids from nonviral protein cages.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- ARiBo pull-down for riboproteomic studies based on label-free quantitative mass spectrometry.RNA (New York, N.Y.) · 2016Article
- Rapid NMR screening of RNA secondary structure and binding.Journal of biomolecular NMR · 2015Article
- Native purification and labeling of RNA for single molecule fluorescence studies.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Progress and outlook in structural biology of large viral RNAs.Virus research · 2014Review
- Advances in methods for native expression and purification of RNA for structural studies.Current opinion in structural biology · 2014Review
- Strong anion-exchange fast performance liquid chromatography as a versatile tool for preparation and purification of RNA produced by in vitro transcription.RNA (New York, N.Y.) · 2013Article
- Affinity purification of T7 RNA transcripts with homogeneous ends using ARiBo and CRISPR tags.RNA (New York, N.Y.) · 2013Article
- Importance of the NCp7-like domain in the recognition of pre-let-7g by the pluripotency factor Lin28.Nucleic acids research · 2012Article
- RNAs synthesized using photocleavable biotinylated nucleotides have dramatically improved catalytic efficiency.Nucleic acids research · 2011Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Although RNA-based biological processes and therapeutics have gained increasing interest, purification of in vitro transcribed RNA generally relies on gel-based methods that are time-consuming, tedious and denature the RNA. Here, we present a reliable procedure for affinity batch purification of RNA, which exploits the high-affinity interaction between the boxB RNA and the N-peptide from bacteriophage λ. The RNA of interest is synthesized with an ARiBo tag, which consists of an activatable ribozyme (the glmS ribozyme) and the λBoxB RNA. This ARiBo-fusion RNA is initially captured on Glutathione-Sepharose resin via a GST/λN-fusion protein, and the RNA of interest is subsequently eluted by ribozyme self-cleavage using glucosamine-6-phosphate. Several GST/λN-fusion proteins and ARiBo tags were tested to optimize RNA yield and purity. The optimized procedure enables one to quickly obtain (3 h) highly pure RNA (>99%) under native conditions and with yields comparable to standard denaturing gel-based protocols. It is widely applicable to a variety of RNAs, including riboswitches, ribozymes and microRNAs. In addition, it can be easily adapted to a wide range of applications that require RNA purification and/or immobilization, including isolation of RNA-associated complexes from living cells and high-throughput applications.
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Registered trials
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