ArticleNucleic acids research2011
Characterization of L1 retrotransposition with high-throughput dual-luciferase assays.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
62 citing papers in PubMed, 102 citations in OpenAlex.
- Cullin 3-mediated ubiquitination restricts enterovirus D68 replication and is counteracted by viral protease 3C.Journal of virology · 2025Article
- SLFN11 Restricts LINE-1 Mobility.Cells · 2025Article
- Memory T-Cell Phenotype in Cutaneous T-Cell Lymphoma Is Modified by Germline Gene Gametocyte Specific Factor 1.Experimental dermatology · 2025Article
- LncRNA-mRNA regulatory network reveals key lncRNAs tightly associated with preterm labor and premature rupture of membranes.Non-coding RNA research · 2025Article
- Identification and characterization of small molecule inhibitors of the LINE-1 retrotransposon endonuclease.Nature communications · 2024Article
- Long interspersed nuclear elements safeguard neural progenitors from precocious differentiation.Cell reports · 2024Article
- Article
- Evolutionary insights from profiling LINE-1 activity at allelic resolution in a single human genome.The EMBO journal · 2024Article
- Article
- ZCCHC3 is a stress granule zinc knuckle protein that strongly suppresses LINE-1 retrotransposition.PLoS genetics · 2023Article
- LINE-1 Retrotransposition Assays in Embryonic Stem Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Sequestration of LINE-1 in cytosolic aggregates by MOV10 restricts retrotransposition.EMBO reports · 2022Article
- Ongoing endeavors to detect mobilization of transposable elements.BMB reports · 2022Review
- Movements of Ancient Human Endogenous Retroviruses Detected in SOX2-Expressing Cells.Journal of virology · 2022Article
- Subfamily-specific differential contribution of individual monomers and the tether sequence to mouse L1 promoter activity.Mobile DNA · 2022Article
- The Zinc-Finger protein ZCCHC3 inhibits LINE-1 retrotransposition.Frontiers in microbiology · 2022Article
- Tracing Mobile DNAs: From Molecular to Population Scales.Frontiers in plant science · 2022Review
- A High Throughput Cell-Based Screen Assay for LINE-1 ORF1p Expression Inhibitors Using the In-Cell Western Technique.Frontiers in pharmacology · 2022Article
- Inhibition of LINE-1 retrotransposition represses telomere reprogramming during mouse 2-cell embryo development.Journal of assisted reproduction and genetics · 2021Article
- Zidovudine inhibits telomere elongation, increases the transposable element LINE-1 copy number and compromises mouse embryo development.Molecular biology reports · 2021Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Recent studies employing genome-wide approaches have provided an unprecedented view of the scope of L1 activities on structural variations in the human genome, and further reinforced the role of L1s as one of the major driving forces behind human genome evolution. The rapid identification of novel L1 elements by these high-throughput approaches demands improved L1 functional assays. However, the existing assays use antibiotic selection markers or fluorescent proteins as reporters; neither is amenable to miniaturization. To increase assay sensitivity and throughput, we have developed a third generation assay by using dual-luciferase reporters, in which firefly luciferase is used as the retrotransposition indicator and Renilla luciferase is encoded on the same or separate plasmid for normalization. This novel assay is highly sensitive and has a broad dynamic range. Quantitative data with high signal-to-noise ratios can be obtained from 24- up to 96-well plates in 2-4 days after transfection. Using the dual-luciferase assays, we have characterized profiles of retrotransposition by various human and mouse L1 elements, and detailed the kinetics of L1 retrotransposition in cultured cells. Its high-throughput and short assay timeframe make it well suited for routine tests as well as large-scale screening efforts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.