ArticleThe EMBO journal1990
Extinction of Ig genes expression in myeloma x fibroblast somatic cell hybrids is accompanied by repression of the oct-2 gene encoding a B-cell specific transcription factor.
Article in The EMBO journal, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 48 citations in OpenAlex.
- Cell fusion in tumor progression: the isolation of cell fusion products by physical methods.Cancer cell international · 2011Article
- Hepatocyte nuclear factor-4 prevents silencing of hepatocyte nuclear factor-1 expression in hepatoma x fibroblast cell hybrids.Nucleic acids research · 1997Article
- E47 activates the Ig-heavy chain and TdT loci in non-B cells.The EMBO journal · 1996Article
- Direct and indirect mechanisms of repression participate in suppression of T-cell-specific gene expression in T x L-cell hybrids.Gene expression · 1996Article
- Differential transactivation potential of Oct1 and Oct2 is determined by additional B cell-specific activities.The EMBO journal · 1994Article
- Negative regulation of transcription in eukaryotes.The Biochemical journal · 1993Review
- Targeted disruption of the Oct-2 locus in a B cell provides genetic evidence for two distinct cell type-specific pathways of octamer element-mediated gene activation.The EMBO journal · 1993Article
- The octamer/mu E4 region of the immunoglobulin heavy-chain enhancer mediates gene repression in myeloma x T-lymphoma hybrids.Molecular and cellular biology · 1993Article
- Extinction of T cell receptor alpha-chain gene expression accompanied by loss of the lymphoid enhancer-binding factor 1 (LEF-1) in murine somatic cell hybrids.Molecular and cellular biology · 1993Article
- Extinction of Oct-3/4 gene expression in embryonal carcinoma x fibroblast somatic cell hybrids is accompanied by changes in the methylation status, chromatin structure, and transcriptional activity of the Oct-3/4 upstream region.Molecular and cellular biology · 1993Article
- Differential induction of transcription factors that regulate the interleukin 2 gene during anergy induction and restimulation.The Journal of experimental medicine · 1992Article
- Regulation and a possible stage-specific function of Oct-2 during pre-B-cell differentiation.Molecular and cellular biology · 1991Article
- The expression of several T cell-specific and novel genes is repressed by trans-acting factors in immature T lymphoma clones.The Journal of experimental medicine · 1991Article
- Extinction of insulin gene expression in hybrids between beta cells and fibroblasts is accompanied by loss of the putative beta-cell-specific transcription factor IEF1.Molecular and cellular biology · 1991Article
- Extinction of retinol-binding protein gene expression in somatic cell-hybrids: identification of the target sequences.Nucleic acids research · 1990Article
- A novel upstream element compensates for an ineffectual octamer motif in an immunoglobulin V kappa promoter.The EMBO journal · 1990Article
- Hepatocyte dedifferentiation and extinction is accompanied by a block in the synthesis of mRNA coding for the transcription factor HNF1/LFB1.The EMBO journal · 1990Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In most instances, fusion of differentiated cell types with fibroblasts has resulted in the extinction of differentiation-specific traits of the nonfibroblast parental cell. To explore the genetic basis of this phenomenon, we have used a series of somatic cell hybrids between myeloma cells and fibroblasts. Previous findings show that in these hybrids expression of the immunoglobulin (Ig) genes was extinguished at the transcriptional level. Our present results show that NF-kappa B transcription factor, known to be critical for kappa-chain enhancer activity, is present although in a lower amount, in the nucleus and in the cytosolic fraction of most of these hybrids (probably attached to the previously postulated I-kappa B inhibitor). In contrast, the expression of the NF-A2/OTF-2 transcription factor encoded by the oct-2 gene, which binds to the octameric motif located in the Ig promoters and heavy chain gene enhancer, is extinguished at the transcriptional level. Our data thus suggest that extinction of Ig genes expression occurs via an indirect mechanism in which a fibroblast factor suppresses transcription factor(s) which are critical for Ig transcription.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.