ArticleTransgenic research2011
Expression of functional recombinant human growth hormone in transgenic soybean seeds.
Article in Transgenic research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 56 citations in OpenAlex.
- Soybean genetic engineering in Brazil and South America - A review.Genetics and molecular biology · 2026Article
- Transgenic Soybean for Production of Thermostable α-Amylase.Plants (Basel, Switzerland) · 2024Article
- Improvement of recombinant miraculin production in transgenic tomato by crossbreeding-based genetic background modification.Transgenic research · 2022Article
- Seed- and leaf-based expression of FGF21-transferrin fusion proteins for oral delivery and treatment of non-alcoholic steatohepatitis.Frontiers in plant science · 2022Article
- Elastin-like polypeptide and γ-zein fusions significantly increase recombinant protein accumulation in soybean seeds.Transgenic research · 2021Article
- Contributions of the international plant science community to the fight against human infectious diseases - part 1: epidemic and pandemic diseases.Plant biotechnology journal · 2021Review
- Evaluation of lettuce chloroplast and soybean cotyledon as platforms for production of functional bone morphogenetic protein 2.Transgenic research · 2019Article
- Russell-Like Bodies in Plant Seeds Share Common Features With Prolamin Bodies and Occur Upon Recombinant Protein Production.Frontiers in plant science · 2019Article
- Improving nutritional quality and fungal tolerance in soya bean and grass pea by expressing an oxalate decarboxylase.Plant biotechnology journal · 2016Article
- Article
- Engineering soya bean seeds as a scalable platform to produce cyanovirin-N, a non-ARV microbicide against HIV.Plant biotechnology journal · 2015Article
- Improved protein quality in transgenic soybean expressing a de novo synthetic protein, MB-16.Transgenic research · 2014Article
- Soybean genetic transformation: A valuable tool for the functional study of genes and the production of agronomically improved plants.Genetics and molecular biology · 2012Article
- NanoUPLC-MSE proteomic data assessment of soybean seeds using the Uniprot database.BMC biotechnology · 2012Article
- Recent advances in soybean transformation and their application to molecular breeding and genomic analysis.Breeding science · 2012Article
- Plant made anti-HIV microbicides--a field of opportunity.Biotechnology advancesReview
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We produced human growth hormone (hGH), a protein that stimulates growth and cell reproduction, in genetically engineered soybean [Glycine max (L.) Merrill] seeds. Utilising the alpha prime (α') subunit of β-conglycinin tissue-specific promoter from soybean and the α-Coixin signal peptide from Coix lacryma-jobi, we obtained transgenic soybean lines that expressed the mature form of hGH in their seeds. Expression levels of bioactive hGH up to 2.9% of the total soluble seed protein content (corresponding to approximately 9 g kg(-1)) were measured in mature dry soybean seeds. The results of ultrastructural immunocytochemistry assays indicated that the recombinant hGH in seed cotyledonary cells was efficiently directed to protein storage vacuoles. Specific bioassays demonstrated that the hGH expressed in the soybean seeds was fully active. The recombinant hGH protein sequence was confirmed by mass spectrometry characterisation. These results demonstrate that the utilisation of tissue-specific regulatory sequences is an attractive and viable option for achieving high-yield production of recombinant proteins in stable transgenic soybean seeds.
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