ArticleJournal of virology2011
Rabies virus (RV) glycoprotein expression levels are not critical for pathogenicity of RV.
Article in Journal of virology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
42 citing papers in PubMed, 67 citations in OpenAlex.
- Green-to-red spectral labeling: A novel polysynaptic retrograde tracing strategy in the marker footprint mouse model.Animal models and experimental medicine · 2025Article
- A Novel Mouse Model for Polysynaptic Retrograde Tracing and Rabies Pathological Research.Cellular and molecular neurobiology · 2023Article
- "Self-inactivating" rabies viruses are susceptible to loss of their intended attenuating modification.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- Recombinant adeno-associated virus serotype 9 AAV-RABVG expressing a Rabies Virus G protein confers long-lasting immune responses in mice and non-human primates.Emerging microbes & infections · 2022Article
- Article
- Newcastle disease virus genotype VII gene expression in experimentally infected birds.Scientific reports · 2022Article
- Altering Compositional Properties of Viral Genomes to Design Live-Attenuated Vaccines.Frontiers in microbiology · 2021Review
- A Bivalent Human Adenovirus Type 5 Vaccine Expressing the Rabies Virus Glycoprotein and Canine Distemper Virus Hemagglutinin Protein Confers Protective Immunity in Mice and Foxes.Frontiers in microbiology · 2020Article
- Amino Acid Mutation in Position 349 of Glycoprotein Affect the Pathogenicity of Rabies Virus.Frontiers in microbiology · 2020Article
- The Deoptimization of Rabies Virus Matrix Protein Impacts Viral Transcription and Replication.Viruses · 2019Article
- Trans-synaptic Neural Circuit-Tracing with Neurotropic Viruses.Neuroscience bulletin · 2019Review
- Article
- Functional brain stem circuits for control of nose motion.Journal of neurophysiology · 2019Article
- Retrograde axonal transport of rabies virus is unaffected by interferon treatment but blocked by emetine locally in axons.PLoS pathogens · 2018Article
- Attenuation of Viruses by Large-Scale Recoding of their Genomes: the Selection Is Always Biased.Current clinical microbiology reports · 2018Review
- Rabies virus modifies host behaviour through a snake-toxin like region of its glycoprotein that inhibits neurotransmitter receptors in the CNS.Scientific reports · 2017Article
- Contribution of Human Lung Parenchyma and Leukocyte Influx to Oxidative Stress and Immune System-Mediated Pathology following Nipah Virus Infection.Journal of virology · 2017Article
- Phenotypic ConsequencesFrontiers in microbiology · 2017Article
- Rabies Virus Infection Induces the Formation of Stress Granules Closely Connected to the Viral Factories.PLoS pathogens · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Previous comparisons of different rabies virus (RV) strains suggested an inverse relationship between pathogenicity and the amount of glycoprotein produced in infected cells. In order to provide more insight into this relationship, we pursued an experimental approach that allowed us to alter the glycoprotein expression level without altering the glycoprotein sequence, thereby eliminating the contribution of amino acid changes to differences in viral virulence. To this end, we constructed an infectious clone of the highly pathogenic rabies virus strain CVS-N2c and replaced its cognate glycoprotein gene with synthetic versions in which silent mutations were introduced to replace wild-type codons with the most or least frequently used synonymous codons. A recombinant N2c variant containing the fully codon-optimized G gene and three variants carrying a partially codon-deoptimized G gene were recovered on mouse neuroblastoma cells and shown to express 2- to 3-fold more and less glycoprotein, respectively, than wild-type N2c. Pathogenicity studies in mice revealed the WT-N2c virus to be the most pathogenic strain. Variants containing partially codon-deoptimized glycoprotein genes or the codon-optimized gene were less pathogenic than WT-N2c but still caused significant mortality. We conclude that the expression level of the glycoprotein gene does have an impact on pathogenicity but is not a dominant factor that determines pathogenicity. Thus, strategies such as changes in codon usage that aim solely at altering the expression level of the glycoprotein gene do not suffice to render a pathogenic rabies virus apathogenic and are not a viable and safe approach for attenuation of a pathogenic strain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.