ArticleAnnals of the rheumatic diseases2011
dsRNA activation of endothelin-1 and markers of vascular activation in endothelial cells and fibroblasts.
Article in Annals of the rheumatic diseases, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- Expansion of Fcγ Receptor IIIa-Positive Macrophages, Ficolin 1-Positive Monocyte-Derived Dendritic Cells, and Plasmacytoid Dendritic Cells Associated With Severe Skin Disease in Systemic Sclerosis.Arthritis & rheumatology (Hoboken, N.J.) · 2022Article
- CirculatoryGenes · 2021Article
- Fever Induced by Zymosan A and Polyinosinic-Polycytidylic Acid in Female Rats: Influence of Sex Hormones and the Participation of Endothelin-1.Inflammation · 2021Article
- Innate Immune Modulation Induced by EBV Lytic Infection Promotes Endothelial Cell Inflammation and Vascular Injury in Scleroderma.Frontiers in immunology · 2021Observational
- Endothelin-1 contributes to the development of virus-induced demyelinating disease.Journal of neuroinflammation · 2020Article
- Toll-like receptors in mediating pathogenesis in systemic sclerosis.Clinical and experimental immunology · 2020Review
- Impact of Immune System Activation and Vascular Impairment on Male and Female Sexual Dysfunction.Sexual medicine reviews · 2019Review
- The role of adipokines in systemic sclerosis: a missing link?Archives of dermatological research · 2019Review
- The Activation of Human Dermal Microvascular Cells by Poly(I:C), Lipopolysaccharide, Imiquimod, and ODN2395 Is Mediated by the Fli1/FOXO3A Pathway.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- Vascular Remodelling and Mesenchymal Transition in Systemic Sclerosis.Stem cells international · 2016Review
- Fresh Insights into Disease Etiology and the Role of Microbial Pathogens.Current rheumatology reports · 2016Review
- Epstein-Barr virus infection induces aberrant TLR activation pathway and fibroblast-myofibroblast conversion in scleroderma.The Journal of investigative dermatology · 2014Article
- Proteome-wide analysis and CXCL4 as a biomarker in systemic sclerosis.The New England journal of medicine · 2014Article
- Hepatitis C virus induced endothelial inflammatory response depends on the functional expression of TNFα receptor subtype 2.PloS one · 2014Article
- The immune pathogenesis of scleroderma: context is everything.Current rheumatology reports · 2013Review
- HLA-B35 and dsRNA induce endothelin-1 via activation of ATF4 in human microvascular endothelial cells.PloS one · 2013Article
- Article
- Endoglin promotes TGF-β/Smad1 signaling in scleroderma fibroblasts.Journal of cellular physiology · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
backgroundIn patients with systemic sclerosis (SSc), the relationship between innate immune activation, represented by increased expression of interferon (IFN)-regulated genes, and vascular injury/activation, manifest by increased endothelin-1 (ET-1), endothelin converting enzyme-1 (ECE1) and intercellular adhesion molecule-1, is uncertain.
objectiveTo investigate the potential roles of innate immune ligands in both these pathogenic pathways.
methodsThe effect of known Toll-like receptor (TLR) ligands was tested in vitro on dermal microvascular and pulmonary arterial endothelial cells, and on dermal fibroblasts cultured from healthy controls and patients with SSc. To test the effect of double-stranded RNA (dsRNA) on vascular activation/injury in vivo, polyinosinic/polycytidylic acid (poly(I:C)) was administered continuously over 7 days by subcutaneous osmotic pump.
resultsdsRNA/poly(I:C), but not other TLR ligands, highly stimulated ET-1 protein and mRNA (EDN1), as well as intercellular adhesion molecule-1 (ICAM-1) and IFN-regulated MX2, by endothelial cells and dermal fibroblasts. Poly(I:C) induced EDN1, ECE1, and ICAM-1 mRNA expression in poly(I:C) treated skin. Poly(I:C)-induced EDN1, ECE1 and MX2 was not blocked in mice with the type I IFN receptor deleted. However, poly(I:C)-induced EDN1 and ECE1, but not poly(I:C)-induced ICAM-1 expression was blocked in mice with the TLR3 signalling protein TRIF/TICAM-1 deleted.
conclusionTogether these data show that the dsRNA can regulate genes associated with vascular activation, as seen in SSc, that type I IFNs do not mediate these effects, and that EDN1 and ECE1 but not ICAM-1 activation is mediated by TLR3.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.