ArticleActa orthopaedica2010
Accelerated fracture healing in mice lacking the 5-lipoxygenase gene.
Article in Acta orthopaedica, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 44 citations in OpenAlex.
- New insights on pharmacological potential of montelukast: a comprehensive review.Inflammopharmacology · 2025Review
- Dose-response effect of Montelukast on post-extraction dental socket repair and skeletal phenotype of mice.Odontology · 2023Article
- Sex-Based Difference in Bone Healing: A Review of Recent Pre-clinical Literature.Current reviews in musculoskeletal medicine · 2022Review
- Alveolar socket healing in 5-lipoxygenase knockout aged female mice treated or not with high dose of zoledronic acid.Scientific reports · 2021Article
- Inhibition of Lipoxygenases Showed No Benefit for the Musculoskeletal System in Estrogen Deficient Rats.Frontiers in endocrinology · 2021Article
- Purification of Soluble Membrane-Bound Ambystoma mexicanum Epidermal Lipoxygenase from E. coli and Its Growth Effect on Human Fetal Foreskin Fibroblast.The protein journal · 2020Article
- Modeling Complex Orthopedic Trauma in Rodents: Bone, Muscle and Nerve Injury and Healing.Frontiers in pharmacology · 2020Review
- New Surgical Model for Bone-Muscle Injury Reveals Age and Gender-Related Healing Patterns in the 5 Lipoxygenase (5LO) Knockout Mouse.Frontiers in endocrinology · 2020Article
- Effect of the lipoxygenase inhibitor baicalein on bone tissue and bone healing in ovariectomized rats.Nutrition & metabolism · 2019Article
- miR-146a suppresses 5-lipoxygenase activating protein (FLAP) expression and Leukotriene B4 production in lung cancer cells.Oncotarget · 2018Article
- Isolated metaphyseal injury influences unrelated bones.Acta orthopaedica · 2017Article
- Salicylic Acid-Based Polymers for Guided Bone Regeneration Using Bone Morphogenetic Protein-2.Tissue engineering. Part A · 2015Article
- Method for measuring lipid mediators, proteins, and messenger RNAs from a single tissue specimen.Analytical biochemistry · 2015Article
- Critical role of 5-lipoxygenase and heme oxygenase-1 in wound healing.The Journal of investigative dermatology · 2014Article
- Immunohistochemical localization of key arachidonic acid metabolism enzymes during fracture healing in mice.PloS one · 2014Article
- Monitoring tissue inflammation and responses to drug treatments in early stages of mice bone fracture using 50 MHz ultrasound.Ultrasonics · 2014Article
- Fracture healing and lipid mediators.BoneKEy reports · 2014Review
- 5-Lipoxygenase facilitates healing after myocardial infarction.Basic research in cardiology · 2013Article
- Local inhibition of 5-lipoxygenase enhances bone formation in a rat model.Bone & joint research · 2013Article
- The genetic profile of bone repair.Clinical cases in mineral and bone metabolism : the official journal of the Italian Society of Osteoporosis, Mineral Metabolism, and Skeletal Diseases · 2013Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
background and purposeCyclooxygenase-2 (COX-2) promotes inflammation by synthesizing pro-inflammatory prostaglandins from arachidonic acid. Inflammation is an early response to bone fracture, and ablation of COX-2 activity impairs fracture healing. Arachidonic acid is also converted into leukotrienes by 5-lipoxygenase (5-LO). We hypothesized that 5-LO is a negative regulator of fracture healing and that in the absence of COX-2, excess leukotrienes synthesized by 5-LO will impair fracture healing.
methodsFracture healing was assessed in mice with a targeted 5-LO mutation (5-LO(KO) mice) and control mice by radiographic and histological observations, and measured by histomorphometry and torsional mechanical testing. To assess effects on arachidonic acid metabolism, prostaglandin E2, F2α, and leukotriene B4 levels were measured in the fracture calluses of control, 5-LO(KO) COX-1(KO), and COX-2(KO) mice by enzyme linked immunoassays.
resultsFemur fractures in 5-LO(KO) mice rapidly developed a cartilaginous callus that was replaced with bone to heal fractures faster than in control mice. Femurs from 5-LO(KO) mice had substantially better mechanical properties after 1 month of healing than did control mice. Callus leukotriene levels were 4-fold higher in mice homozygous for a targeted mutation in the COX-2 gene (COX-2(KO)), which indicated that arachidonic acid was shunted into the 5-LO pathway in the absence of COX-2.
interpretationThese experiments show that 5-LO negatively regulates fracture healing and that shunting of arachidonic acid into the 5-LO pathway may account, at least in part, for the impaired fracture healing response observed in COX-2(KO) mice.
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