ArticleJournal of insect science (Online)2010
Why does insect RNA look degraded?
Article in Journal of insect science (Online), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed.
- Rearing, dissection, and temporal transcriptomic profiling protocols to study density-dependent phenotypic plasticity in the genus Schistocerca.STAR protocols · 2026Article
- Protocol for preparation of a synaptosome-enriched fraction to profile presynaptic RNA content in adult Drosophila brains.STAR protocols · 2026Article
- The venom gland transcriptome of Tityus paraguayensis reveals a diverse array of bioactive molecules from the Brazilian Cerrado.PloS one · 2026Article
- Article
- Convergent Molecular Evolution Associated With Repeated Transitions to Gregarious Larval Behavior in Heliconiini.Molecular biology and evolution · 2025Article
- RNA Sequencing Dataset ofData · 2025Article
- Improved RNA preparation for RNA-seq of the intracellular bacteriumFrontiers in microbiology · 2025Article
- Evidence for the replication of a plant rhabdovirus in its arthropod mite vector.Virus research · 2025Article
- EFFICIENT RIBOSOMAL RNA DEPLETION FROMWellcome open research · 2025Article
- Sex-limited experimental evolution drives transcriptomic divergence in a hermaphrodite.Genome biology and evolution · 2024Article
- MicroRNAs are deeply linked to the emergence of the complex octopus brain.Science advances · 2022Article
- Honey Bee Pathogen Prevalence and Interactions within the Marmara Region of Turkey.Veterinary sciences · 2022Article
- Genome assembly and annotation of the European earwig Forficula auricularia (subspecies B).G3 (Bethesda, Md.) · 2022Article
- An RNA-immunoprecipitation protocol to identify RNAs associated with RNA-binding proteins in cytoplasmic and nuclearSTAR protocols · 2022Article
- Typical structure of rRNA coding genes in diplonemids points to two independent origins of the bizarre rDNA structures of euglenozoans.BMC ecology and evolution · 2022Article
- Transcriptomic Analysis of the Venom Gland and Enzymatic Characterization of the Venom ofToxins · 2022Article
- Assessment of RNA extraction protocols from cladocerans.PloS one · 2022Article
- MicroRNA profile of the strobilated worms of Echinococcus granulosus derived from in vivo and in vitro systems by using high-throughput approach.Parasitology research · 2021Article
- ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics.Genome biology · 2021Article
- Transcriptomic Responses of the Honey Bee Brain to Infection with Deformed Wing Virus.Viruses · 2021Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The integrity of extracted ribonucleic acid (RNA) is commonly assessed by gel electrophoresis and subsequent analysis of the ribosomal RNA (rRNA) bands. Using the honey bee, Apis mellifera (Hymenoptera: Apidae), as an example, the electrophoretic rRNA profile of insects is explained. This profile differs significantly from the standard benchmark since the 28S rRNA of most insects contains an endogenous "hidden break." Upon denaturation, the masking hydrogen bonds are disrupted, releasing two similar sized fragments that both migrate closely with 18S rRNA. The resulting rRNA profile thus reflects the endogenous composition of insect rRNA and should not be misinterpreted as degradation.
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