ReviewProtein science : a publication of the Protein Society2011
Converting a protein into a switch for biosensing and functional regulation.
Review in Protein science : a publication of the Protein Society, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- Triplex DNA clamp regulates Cas12a activation for ssDNA and RNA sensing.Nucleic acids research · 2026Article
- Seesaw protein: Design of a protein that adopts interconvertible alternative functional conformations and its dynamics.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Enhancing response of a protein conformational switch by using two disordered ligand binding domains.Frontiers in molecular biosciences · 2023Article
- Engineering a Fluorescent Protein Color Switch Using Entropy-Driven β-Strand Exchange.ACS sensors · 2022Article
- Engineering protein and DNA tools for creating DNA-dependent protein switches.Methods in enzymology · 2022Article
- The role of C-terminal helix in the conformational transition of an arginine binding protein.Journal of structural biology: X · 2022Article
- Influence of Secondary-Structure Folding on the Mutually Exclusive Folding Process of GL5/I27 Protein: Evidence from Molecular Dynamics Simulations.International journal of molecular sciences · 2016Article
- The interplay between effector binding and allostery in an engineered protein switch.Protein science : a publication of the Protein Society · 2016Article
- Electrochemical activation of engineered protein switches.Biotechnology and bioengineering · 2016Article
- A critical element of the light-induced quaternary structural changes in YtvA-LOV.Protein science : a publication of the Protein Society · 2015Article
- A Modular, DNA-Based Beacon for Single-Step Fluorescence Detection of Antibodies and Other Proteins.Angewandte Chemie (International ed. in English) · 2015Article
- Design of protein switches based on an ensemble model of allostery.Nature communications · 2015Article
- Design of catalytically amplified sensors for small molecules.Biomolecules · 2014Review
- Thermodynamic basis for engineering high-affinity, high-specificity binding-induced DNA clamp nanoswitches.ACS nano · 2013Article
- The designability of protein switches by chemical rescue of structure: mechanisms of inactivation and reactivation.Journal of the American Chemical Society · 2013Article
- Tuning response curves for synthetic biology.ACS synthetic biology · 2013Article
- GFP variants with alternative β-strands and their application as light-driven protease sensors: a tale of two tails.Journal of the American Chemical Society · 2013Article
- Bioelectrochemical switches for the quantitative detection of antibodies directly in whole blood.Journal of the American Chemical Society · 2012Article
- Designing redox potential-controlled protein switches based on mutually exclusive proteins.Protein science : a publication of the Protein Society · 2012Article
- Re-engineering electrochemical biosensors to narrow or extend their useful dynamic range.Angewandte Chemie (International ed. in English) · 2012Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Proteins that switch conformations in response to a signaling event (e.g., ligand binding or chemical modification) present a unique solution to the design of reagent-free biosensors as well as molecules whose biological functions are regulated in useful ways. The principal roadblock in the path to develop such molecules is that the majority of natural proteins do not change conformation upon binding their cognate ligands or becoming chemically modified. Herein, we review recent protein engineering efforts to introduce switching properties into binding proteins. By co-opting natural allosteric coupling, joining proteins in creative ways and formulating altogether new switching mechanisms, researchers are learning how to coax conformational changes from proteins that previously had none. These studies are providing some answers to the challenging question: how can one convert a lock-and-key binding protein into a molecular switch?
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.