ArticleJournal of bioenergetics and biomembranes2010
Estimation of the electric plasma membrane potential difference in yeast with fluorescent dyes: comparative study of methods.
Article in Journal of bioenergetics and biomembranes, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Who cites it
18 citing papers in PubMed.
- From the Metabolic Effects and Mechanism of Monovalent Cation Transport to the Actual Measurement of the Plasma Membrane Potential in Yeast.Journal of fungi (Basel, Switzerland) · 2025Review
- Fluorescent Probe as Dual-Organelle Localizer Through Differential Proton Gradients Between Lipid Droplets and Mitochondria.Analytical chemistry · 2024Article
- The Use of Thioflavin T for the Estimation and Measurement of the Plasma Membrane Electric Potential Difference in Different Yeast Strains.Journal of fungi (Basel, Switzerland) · 2023Article
- Article
- Involvement of multiple influx and efflux transporters in the accumulation of cationic fluorescent dyes by Escherichia coli.BMC microbiology · 2019Article
- Putative role of an SLC45 HPflugers Archiv : European journal of physiology · 2017Article
- Acridine yellow. A novel use to estimate and measure the plasma membrane potential in Saccharomyces cerevisiae.Journal of bioenergetics and biomembranes · 2017Article
- Electron transport chain in a thermotolerant yeast.Journal of bioenergetics and biomembranes · 2017Article
- High Osmolarity Environments Activate the Mitochondrial Alternative Oxidase in Debaryomyces Hansenii.PloS one · 2017Article
- Quantitative description of ion transport via plasma membrane of yeast and small cells.Frontiers in plant science · 2015Review
- Characterization of the effects of a polyunsaturated fatty acid (PUFA) on mitochondrial bioenergetics of chronologically aged yeast.Journal of bioenergetics and biomembranes · 2014Article
- Complementary Methods of Processing diS-C3(3) Fluorescence Spectra Used for Monitoring the Plasma Membrane Potential of Yeast: Their Pros and Cons.Journal of fluorescence · 2014Article
- Early changes in membrane potential of Saccharomyces cerevisiae induced by varying extracellular K(+), Na (+) or H (+) concentrations.Journal of bioenergetics and biomembranes · 2013Article
- Kch1 family proteins mediate essential responses to endoplasmic reticulum stresses in the yeasts Saccharomyces cerevisiae and Candida albicans.The Journal of biological chemistry · 2013Article
- Cell dualism: presence of cells with alternative membrane potentials in growing populations of bacteria and yeasts.Journal of bioenergetics and biomembranes · 2013Article
- Activation of an essential calcium signaling pathway in Saccharomyces cerevisiae by Kch1 and Kch2, putative low-affinity potassium transporters.Eukaryotic cell · 2013Article
- Effects of chitosan on Candida albicans: conditions for its antifungal activity.BioMed research international · 2013Article
- Monitoring of real changes of plasma membrane potential by diS-C(3)(3) fluorescence in yeast cell suspensions.Journal of bioenergetics and biomembranes · 2012Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Different methods to estimate the plasma membrane potential difference (PMP) of yeast cells with fluorescent monitors were compared. The validity of the methods was tested by the fluorescence difference with or without glucose, and its decrease by the addition of 10 mM KCl. Low CaCl₂ concentrations avoid binding of the dye to the cell surface, and low CCCP concentrations avoid its accumulation by mitochondria. Lower concentrations of Ba²+ produce a similar effect as Ca²+, without producing the fluorescence changes derived from its transport. Fluorescence changes without considering binding of the dyes to the cells and accumulation by mitochondria are overshadowed by their distribution between this organelle and the cytoplasm. Other factors, such as yeast starvation, dye used, parameters of the fluorescence changes, as well as buffers and incubation times were analyzed. An additional approach to measure the actual or relative values of PMP, determining the accumulation of the dye, is presented.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.