ArticleHistochemistry and cell biology2010
Comparison of methods of high-pressure freezing and automated freeze-substitution of suspension cells combined with LR White embedding.
Article in Histochemistry and cell biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Immunoelectron microscopy: a comprehensive guide from sample preparation to high-resolution imaging.Discover nano · 2025Review
- Electron Microscopy Analysis of Membraneless Condensates in Plant Cells.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Arabidopsis HOPS subunit VPS41 carries out plant-specific roles in vacuolar transport and vegetative growth.Plant physiology · 2022Article
- Colocalization coefficients evaluating the distribution of molecular targets in microscopy methods based on pointed patterns.Histochemistry and cell biology · 2016Article
- Quantitative evaluation of freeze-substitution effects on preservation of nuclear antigens during preparation of biological samples for immunoelectron microscopy.Histochemistry and cell biology · 2012Article
- Histochemistry and cell biology: the annual review 2010.Histochemistry and cell biology · 2011Review
- UBF complexes with phosphatidylinositol 4,5-bisphosphate in nucleolar organizer regions regardless of ongoing RNA polymerase I activity.Nucleus (Austin, Tex.)Article
Corrections and comments
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Authors and funding
3 authors.
Funding
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Abstract
In this study we present an optimized method of high-pressure freezing and automated freeze-substitution of cultured human cells, followed by LR White embedding, for subsequent immunolabeling. Also, the influence of various conditions of the freeze-substitution procedures such as temperature, duration, and additives in the substitution medium on the preservation of cryo-immobilized cells was analyzed. The recommended approach combines (1) automated freeze-substitution for high reproducibility and minimizing human-derived errors; (2) minimal addition of contrasting and fixing agents; (3) easy-to-use LR White resin for embedment; (4) good preservation of nuclei and nucleoli which are usually the most difficult structures to effectively vitrify and saturate in a resin; and (5) preservation of antigens for sensitive immunogold labeling.
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Registered trials
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