ArticleNucleic acids research2011
A nucleoside kinase as a dual selector for genetic switches and circuits.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Leveraging Autonomous Biological Oscillators for Directed Evolution of Switchable Proteins.Advances in experimental medicine and biology · 2026Review
- Divergent directed evolution of a TetR-type repressor towards aromatic molecules.Nucleic acids research · 2023Article
- Characterization of ColE1 Production for RobustACS synthetic biology · 2022Article
- Transcription Factors as Evolvable Biosensors.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2021Review
- Robust and flexible platform for directed evolution of yeast genetic switches.Nature communications · 2021Article
- Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors.Nature biotechnology · 2020Article
- Escherichia coli "Marionette" strains with 12 highly optimized small-molecule sensors.Nature chemical biology · 2019Article
- Long-range transcriptional interference in E. coli used to construct a dual positive selection system for genetic switches.Nucleic acids research · 2016Article
- A set of powerful negative selection systems for unmodified Enterobacteriaceae.Nucleic acids research · 2015Article
- Rapid and liquid-based selection of genetic switches using nucleoside kinase fused with aminoglycoside phosphotransferase.PloS one · 2015Article
- Article
- Rational optimization of tolC as a powerful dual selectable marker for genome engineering.Nucleic acids research · 2014Article
- Directed evolution as a powerful synthetic biology tool.Methods (San Diego, Calif.) · 2013Article
- Manipulating replisome dynamics to enhance lambda Red-mediated multiplex genome engineering.Nucleic acids research · 2012Article
- Directed evolution: an evolving and enabling synthetic biology tool.Current opinion in chemical biology · 2012Review
- A genetic bistable switch utilizing nonlinear protein degradation.Journal of biological engineering · 2012Article
- Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The development of genetic switches and their integrated forms (genetic circuits) with desired specifications/functions is key for success in synthetic biology. Due to the difficulty in rational design, genetic switches and circuits with desirable specifications are mostly obtained by directed evolution. Based on a virus-derived nucleotide kinase as a single-gene dual selector, we constructed a robust, efficient and stringent selection system for genetic switches. This method exhibited unprecedented enrichment efficacy (>30,000-fold) of functional switches from non-functional ones in a single selection cycle. In addition, negative (OFF) selection was exceptionally stringent, allowing the rapid and efficient selection of non-leaky from leaky circuits.
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