ArticleThe Journal of biological chemistry2011
Manganese binds to Clostridium difficile Fbp68 and is essential for fibronectin binding.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.
- Antibiotic-Associated Diarrhea BeyondThe Brown journal of hospital medicine · 2023Pooled it
- Article
- Article
- Review
- Binding of the extracellular matrix laminin-1 to Clostridioides difficile strains.Memorias do Instituto Oswaldo Cruz · 2022Article
- Foes or Friends? Bacteria Enriched in the Tumor Microenvironment of Colorectal Cancer.Cancers · 2020Review
- Characterization of Highly Mucus-Adherent Non-GMO Derivatives ofFrontiers in bioengineering and biotechnology · 2020Article
- TargetingFrontiers in microbiology · 2018Review
- Characteristics of the Clostridium difficile cell envelope and its importance in therapeutics.Microbial biotechnology · 2017Review
- Clostridium difficile infection.Nature reviews. Disease primers · 2016Review
- Complete genome sequence ofGut pathogens · 2016Article
- Article
- A novel secreted metalloprotease (CD2830) from Clostridium difficile cleaves specific proline sequences in LPXTG cell surface proteins.Molecular & cellular proteomics : MCP · 2014Article
- Clostridium difficile toxin CDT hijacks microtubule organization and reroutes vesicle traffic to increase pathogen adherence.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Clostridium difficile virulence factors: Insights into an anaerobic spore-forming pathogen.Gut microbes · 2014Review
- A highly conserved family of domains related to the DNA-glycosylase fold helps predict multiple novel pathways for RNA modifications.RNA biology · 2014Article
- Elastin, a novel extracellular matrix protein adhering to mycobacterial antigen 85 complex.The Journal of biological chemistry · 2013Article
- Novel mycobacteria antigen 85 complex binding motif on fibronectin.The Journal of biological chemistry · 2012Article
- Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Clostridium difficile is an etiological agent of pseudomembranous colitis and antibiotic-associated diarrhea. Adhesion is the crucial first step in bacterial infection. Thus, in addition to toxins, the importance of colonization factors in C. difficile-associated disease is recognized. In this study, we identified Fbp68, one of the colonization factors that bind to fibronectin (Fn), as a manganese-binding protein (K(D) = 52.70 ± 1.97 nM). Furthermore, the conformation of Fbp68 changed dramatically upon manganese binding. Manganese binding can also stabilize the structure of Fbp68 as evidenced by the increased T(m) measured by thermodenatured circular dichroism and differential scanning calorimetry (CD, T(m) = 58-65 °C; differential scanning calorimetry, T(m) = 59-66 °C). In addition, enhanced tolerance to protease K also suggests greatly improved stability of Fbp68 through manganese binding. Fn binding activity was found to be dependent on manganese due to the lack of binding by manganese-free Fbp68 to Fn. The C-terminal 194 amino acid residues of Fbp68 (Fbp68C) were discovered to bind to the N-terminal domain of Fn (Fbp68C-NTD, K(D) = 233 ± 10 nM, obtained from isothermal titration calorimetry). Moreover, adhesion of C. difficile to Caco-2 cells can be partially blocked if cells are pretreated with Fbp68C, and the binding of Fbp68C on Fn siRNA-transfected cells was significantly reduced. These results raise the possibility that Fbp68 plays a key role in C. difficile adherence on host cells to initiate infection.
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Registered trials
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