ArticleBiochemistry2010
Cellular uptake of ribonuclease A relies on anionic glycans.
Article in Biochemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 42 citations in OpenAlex.
- Preparation, expression, and anti-cancer effects of the novel fusion protein rONC-T7-pHLIP.Applied microbiology and biotechnology · 2025Article
- Bovine Pancreatic RNase A: An Insight into the Mechanism of Antitumor Activity In Vitro and In Vivo.Pharmaceutics · 2022Article
- Discovery of antitumor effects of leczymes.Glycoconjugate journal · 2022Review
- Ribonuclease zymogen induces cytotoxicity upon HIV-1 infection.AIDS research and therapy · 2021Article
- Strengths and Challenges of Secretory Ribonucleases as AntiTumor Agents.Pharmaceutics · 2021Review
- Antimicrobial Synergy of a Ribonuclease and a Peptide Secreted by Human Cells.ACS infectious diseases · 2020Article
- A bispecific circular aptamer tethering a built-in universal molecular tag for functional protein delivery.Chemical science · 2020Article
- A Human Ribonuclease Variant and ERK-Pathway Inhibitors Exhibit Highly Synergistic Toxicity for Cancer Cells.Molecular cancer therapeutics · 2018Article
- Efficient Assembly of Quantum Dots with Homogenous Glycans Derived from Natural N-Linked Glycoproteins.Bioconjugate chemistry · 2018Article
- Comparative functional analysis of ribonuclease 1 homologs: molecular insights into evolving vertebrate physiology.The Biochemical journal · 2017Article
- Knockout of the Ribonuclease Inhibitor Gene Leaves Human Cells Vulnerable to Secretory Ribonucleases.Biochemistry · 2016Article
- Human Cancer Antigen Globo H Is a Cell-Surface Ligand for Human Ribonuclease 1.ACS central science · 2015Article
- Bovine brain ribonuclease is the functional homolog of human ribonuclease 1.The Journal of biological chemistry · 2014Article
- Functional evolution of ribonuclease inhibitor: insights from birds and reptiles.Journal of molecular biology · 2014Article
- Sialyl-glycoconjugates in cholesterol-rich microdomains of P388 cells are the triggers for apoptosis induced by Rana catesbeiana oocyte ribonuclease.Glycoconjugate journal · 2014Article
- Contribution of electrostatics to the binding of pancreatic-type ribonucleases to membranes.Biochemistry · 2013Article
- Human ribonuclease with a pendant poly(ethylene glycol) inhibits tumor growth in mice.Translational oncology · 2013Article
- Fluorogenic probe for constitutive cellular endocytosis.Chemistry & biology · 2013Article
- Fluorogenic label to quantify the cytosolic delivery of macromolecules.Molecular bioSystems · 2013Article
- Interaction of nucleic acids with the glycocalyx.Journal of the American Chemical Society · 2012Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Bovine pancreatic ribonuclease (RNase A) can enter human cells, even though it lacks a cognate cell-surface receptor protein. Here, we report on the biochemical basis for its cellular uptake. Analyses in vitro and in cellulo revealed that RNase A interacts tightly with abundant cell-surface proteoglycans containing glycosaminoglycans, such as heparan sulfate and chondroitin sulfate, as well as with sialic acid-containing glycoproteins. The uptake of RNase A correlates with cell anionicity, as quantified by measuring electrophoretic mobility. The cellular binding and uptake of RNase A contrast with those of Onconase, an amphibian homologue that does not interact tightly with anionic cell-surface glycans. As anionic glycans are especially abundant on human tumor cells, our data predicate utility for mammalian ribonucleases as cancer chemotherapeutic agents.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.