ArticleAnalytical chemistry2010
Activated-ion electron transfer dissociation improves the ability of electron transfer dissociation to identify peptides in a complex mixture.
Article in Analytical chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Implementation of Infrared-Activated Negative Electron Transfer Dissociation (IR-NETD) Using Xenon on a Quadrupole-Orbitrap-Quadrupole Linear Ion Trap Mass Spectrometer.Journal of the American Society for Mass Spectrometry · 2026Article
- Instrumentation at the Leading Edge of Proteomics.Analytical chemistry · 2024Review
- Characterization of an Omnitrap-Orbitrap Platform Equipped with Infrared Multiphoton Dissociation, Ultraviolet Photodissociation, and Electron Capture Dissociation for the Analysis of Peptides and Proteins.Analytical chemistry · 2023Article
- N-glycoproteomics of brain synapses and synaptic vesicles.Cell reports · 2023Article
- Practical Effects of Intramolecular Hydrogen Rearrangement in Electron Transfer Dissociation-Based Proteomics.Journal of the American Society for Mass Spectrometry · 2022Article
- Coupling 193 nm Ultraviolet Photodissociation and Ion Mobility for Sequence Characterization of Conformationally-Selected Peptides.Journal of the American Society for Mass Spectrometry · 2020Article
- Mapping Physiological ADP-Ribosylation Using Activated Ion Electron Transfer Dissociation.Cell reports · 2020Article
- Optical Fiber-Enabled Photoactivation of Peptides and Proteins.Analytical chemistry · 2020Article
- Top-Down Characterization of an Intact Monoclonal Antibody Using Activated Ion Electron Transfer Dissociation.Analytical chemistry · 2020Article
- UV-Vis Photodissociation Action Spectroscopy on Thermo LTQ-XL ETD and Bruker amaZon Ion Trap Mass Spectrometers: a Practical Guide.Journal of the American Society for Mass Spectrometry · 2019Article
- Interactive Peptide Spectral Annotator: A Versatile Web-based Tool for Proteomic Applications.Molecular & cellular proteomics : MCP · 2019Article
- DecoyDeveloper: An On-Demand, De Novo Decoy Glycopeptide Generator.Journal of proteome research · 2019Article
- Top-Down Characterization of Proteins with Intact Disulfide Bonds Using Activated-Ion Electron Transfer Dissociation.Analytical chemistry · 2018Article
- The Value of Activated Ion Electron Transfer Dissociation for High-Throughput Top-Down Characterization of Intact Proteins.Analytical chemistry · 2018Article
- The Role of Electron Transfer Dissociation in Modern Proteomics.Analytical chemistry · 2018Review
- Sequencing Larger Intact Proteins (30-70 kDa) with Activated Ion Electron Transfer Dissociation.Journal of the American Society for Mass Spectrometry · 2018Article
- Sulfur Pentafluoride is a Preferred Reagent Cation for Negative Electron Transfer Dissociation.Journal of the American Society for Mass Spectrometry · 2017Article
- Phosphoproteomics with Activated Ion Electron Transfer Dissociation.Analytical chemistry · 2017Article
- Implementation of Activated Ion Electron Transfer Dissociation on a Quadrupole-Orbitrap-Linear Ion Trap Hybrid Mass Spectrometer.Analytical chemistry · 2017Article
- Where Does the Electron Go? Stable and Metastable Peptide Cation Radicals Formed by Electron Transfer.Journal of the American Society for Mass Spectrometry · 2017Article
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Authors and funding
8 authors.
Funding
Abstract
Using a modified electron transfer dissociation (ETD)-enabled quadrupole linear ion trap (QLT) mass spectrometer, we demonstrate the utility of IR activation concomitant with ETD ion-ion reactions (activated-ion ETD, AI-ETD). Analyzing 12 strong cation exchanged (SCX) fractions of a LysC digest of human cell protein extract using ETD, collision-activated dissociation (CAD), and AI-ETD, we find that AI-ETD generates 13 405 peptide spectral matches (PSMs) at a 1% false-discovery rate (1% FDR), surpassing both ETD (7 968) and CAD (10 904). We also analyze 12 SCX fractions of a tryptic digest of human cell protein extract and find that ETD produces 6 234 PSMs, AI-ETD 9 130 PSMs, and CAD 15 209 PSMs. Compared to ETD with supplemental collisional activation (ETcaD), AI-ETD generates ∼80% more PSMs for the whole cell lysate digested with trypsin and ∼50% more PSMs for the whole cell lysate digested with LysC.
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Registered trials
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