ArticleAnalytical chemistry2010
Ultrasensitive and high-throughput fluorescence analysis of droplet contents with orthogonal line confocal excitation.
Article in Analytical chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed.
- Content Size-Dependent Alginate Microcapsule Formation Using Centrifugation to Eliminate Empty Microcapsules for On-Chip Imaging Cell Sorter Application.Micromachines · 2022Article
- Learning from droplet flows in microfluidic channels using deep neural networks.Scientific reports · 2019Article
- Review
- Single cell kinase signaling assay using pinched flow coupled droplet microfluidics.Biomicrofluidics · 2014Article
- The potential impact of droplet microfluidics in biology.Analytical chemistry · 2013Article
- An automated high-throughput counting method for screening circulating tumor cells in peripheral blood.Analytical chemistry · 2013Article
- A droplet-based, optofluidic device for high-throughput, quantitative bioanalysis.Analytical chemistry · 2012Article
- Sensitive and high-throughput isolation of rare cells from peripheral blood with ensemble-decision aliquot ranking.Angewandte Chemie (International ed. in English) · 2012Article
- Total internal reflection fluorescence microscopy imaging-guided confocal single-molecule fluorescence spectroscopy.The Review of scientific instruments · 2012Article
- Single-monomer formulation of polymerized polyethylene glycol diacrylate as a nonadsorptive material for microfluidics.Analytical chemistry · 2011Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
This paper describes a simple modification to traditional confocal fluorescence detection that greatly improves signal-to-noise (s/n) for the high-speed analysis of droplet streams. Rather than using the conventional epi geometry, illumination of the droplet was in the form of a line that is orthogonal to both the direction of flow and the light-collection objective. In contrast to the epi geometry where we observed high levels of scattering background from the droplets, we detected more than 10-fold less background (depending on the laser power used) when orthogonal-line-confocal illumination was used. We characterized this improvement using a standard microfluidic platform over a range of analyte concentrations and observed an improvement in limits of detection of greater than 10. Using this method, we were able to analyze picomolar concentrations of analytes contained within picoliter-volume droplets at a rate of greater than 350 droplets per second.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.