ArticleCellular and molecular neurobiology2010
Polarized TIRFM reveals changes in plasma membrane topology before and during granule fusion.
Article in Cellular and molecular neurobiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- Illuminating cellular architecture and dynamics with fluorescence polarization microscopy.Journal of cell science · 2024Review
- OOPS: Object-Oriented Polarization Software for analysis of fluorescence polarization microscopy images.PLoS computational biology · 2024Article
- Imaging nanoscale axial dynamics at the basal plasma membrane.The international journal of biochemistry & cell biology · 2023Review
- Imaging Membrane Curvature inside a FcεRI-Centric Synapse in RBL-2H3 Cells Using TIRF Microscopy with Polarized Excitation.Journal of imaging · 2019Article
- Chromogranin A, the major lumenal protein in chromaffin granules, controls fusion pore expansion.The Journal of general physiology · 2019Article
- The fusion pore, 60 years after the first cartoon.FEBS letters · 2018Review
- Distinct patterns of exocytosis elicited by CaPflugers Archiv : European journal of physiology · 2018Article
- Membrane bending occurs at all stages of clathrin-coat assembly and defines endocytic dynamics.Nature communications · 2018Article
- Nanoscale Membrane Budding Induced by CTxB and Detected via Polarized Localization Microscopy.Biophysical journal · 2017Article
- The Detection of Nanoscale Membrane Bending with Polarized Localization Microscopy.Biophysical journal · 2017Article
- Slow fusion pore expansion creates a unique reaction chamber for co-packaged cargo.The Journal of general physiology · 2017Article
- Imaging plasma membrane deformations with pTIRFM.Journal of visualized experiments : JoVE · 2014Article
- Polarization-controlled TIRFM with focal drift and spatial field intensity correction.Biophysical journal · 2014Article
- Platelet granule exocytosis: a comparison with chromaffin cells.Frontiers in endocrinology · 2013Article
- Real-time imaging of plasma membrane deformations reveals pre-fusion membrane curvature changes and a role for dynamin in the regulation of fusion pore expansion.Journal of neurochemistry · 2012Review
- Role of PI(4,5)P(2) in vesicle exocytosis and membrane fusion.Sub-cellular biochemistry · 2012Review
- A new role for the dynamin GTPase in the regulation of fusion pore expansion.Molecular biology of the cell · 2011Article
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Authors and funding
3 authors at 1 institution in 1 country.
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Abstract
We have recently developed a combination of polarization and total internal reflection fluorescence microscopy (pTIRFM) to monitor changes in plasma membrane topology occurring after fusion of chromaffin granules. In this report, pTIRFM is further exploited to reveal two major findings in regards to the secretory pathway in bovine chromaffin cells. First, we show that changes in membrane topology are sometimes detected even prior to fusion. This occurs with high probability in a small subset of granules that appear in the evanescent field during the experiment. On these occasions, the plasma membrane invaginates with the movement just preceding the appearance of a granule in the evanescent field. Such events may represent a direct interaction of the granule with the plasma membrane. Second, we show that the topological fate of the post-fusion, granule/plasma membrane intermediate is regulated by divalent cation. When Sr2+ is used instead of Ca2+ to trigger exocytosis, membrane topology in the exocytotic region is stabilized with significant curvature and indentation.
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