ArticlePloS one2010
Global analysis of circulating immune cells by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Immunoproteomics: The Evolution from Classical Methods to Emerging Techniques.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Cell-Main Spectra Profile Screening Technique in Simulation of Circulating Tumour Cells Using MALDI-TOF Mass Spectrometry.Cancers · 2021Article
- Whole Cell MALDI Fingerprinting Is a Robust Tool for Differential Profiling of Two-Component Mammalian Cell Mixtures.Journal of the American Society for Mass Spectrometry · 2019Article
- MALDI-TOF MS and point of care are disruptive diagnostic tools in Africa.New microbes and new infections · 2018Article
- MALDI-TOF MS monitoring of PBMC activation status in sepsis.BMC infectious diseases · 2018Article
- Rapid and robust MALDI-TOF MS techniques for microbial identification: a brief overview of their diverse applications.Journal of microbiology (Seoul, Korea) · 2018Review
- Ratiometric Mass Spectrometry for Cell Identification and Quantitation Using Intracellular "Dual-Biomarkers".Scientific reports · 2017Article
- Intact Cell MALDI-TOF MS on Sperm: A Molecular Test For Male Fertility Diagnosis.Molecular & cellular proteomics : MCP · 2016Article
- Quantitative whole-cell MALDI-TOF MS fingerprints distinguishes human monocyte sub-populations activated by distinct microbial ligands.BMC biotechnology · 2015Article
- Phenotypic diversity and emerging new tools to study macrophage activation in bacterial infectious diseases.Frontiers in immunology · 2014Review
- MAP1S controls breast cancer cell TLR5 signaling pathway and promotes TLR5 signaling-based tumor suppression.PloS one · 2014Article
- Whole-cell MALDI-TOF mass spectrometry is an accurate and rapid method to analyze different modes of macrophage activation.Journal of visualized experiments : JoVE · 2013Article
- Identification of Tsetse (Glossina spp.) using matrix-assisted laser desorption/ionisation time of flight mass spectrometry.PLoS neglected tropical diseases · 2013Article
- Classification of ancient mammal individuals using dental pulp MALDI-TOF MS peptide profiling.PloS one · 2011Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMALDI-TOF mass spectrometry is currently used in microbiological diagnosis to characterize bacterial populations. Our aim was to determine whether this technique could be applied to intact eukaryotic cells, and in particular, to cells involved in the immune response. METHODOLOGY/PRINCIPAL
findingsA comparison of frozen monocytes, T lymphocytes and polymorphonuclear leukocytes revealed specific peak profiles. We also found that twenty cell types had specific profiles, permitting the establishment of a cell database. The circulating immune cells, namely monocytes, T lymphocytes and polymorphonuclear cells, were distinct from tissue immune cells such as monocyte-derived macrophages and dendritic cells. In addition, MALDI-TOF mass spectrometry was valuable to easily identify the signatures of monocytes and T lymphocytes in peripheral mononuclear cells. CONCLUSIONS/SIGNIFICANCE: This method was rapid and easy to perform, and unlike flow cytometry, it did not require any additional components such as specific antibodies. The MALDI-TOF mass spectrometry approach could be extended to analyze the cell composition of tissues and the activation state of immune cells.
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