ArticlePloS one2010
Diclofenac hypersensitivity: antibody responses to the parent drug and relevant metabolites.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 56 citations in OpenAlex.
- Ex vivo Drug Metabolism Using Primary Human Hepatocytes for the Detection of Metabolite-Dependent Antibodies to Red Blood Cells.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2025Article
- Updating the classification and routine diagnosis of NSAID hypersensitivity reactions: A WAO Statement.The World Allergy Organization journal · 2025Review
- Diclofenac Immune-Mediated Hepatitis: Identification of Innate and Adaptive Immune Responses at Clinically Relevant Doses.International journal of molecular sciences · 2025Article
- Re-assessment of monoclonal antibodies against diclofenac for their application in the analysis of environmental waters.Analytical methods : advancing methods and applications · 2024Article
- Diclofenac Disrupts the Circadian Clock and through Complex Cross-Talks Aggravates Immune-Mediated Liver Injury-A Repeated Dose Study in Minipigs for 28 Days.International journal of molecular sciences · 2023Article
- A rapid magnetic bead-based immunoassay for sensitive determination of diclofenac.Analytical and bioanalytical chemistry · 2022Article
- Evaluation of Subjects Experiencing Allergic Reactions to Non-Steroidal Anti-Inflammatory Drugs: Clinical Characteristics and Drugs Involved.Frontiers in pharmacology · 2020Article
- Polymorphisms in CEP68 gene associated with risk of immediate selective reactions to non-steroidal anti-inflammatory drugs.The pharmacogenomics journal · 2019Article
- BAT in the Diagnosis of Drug Allergy: a Novel Tool in Clinical Daily Practice?Current allergy and asthma reports · 2019Review
- NSAID-induced reactions: classification, prevalence, impact, and management strategies.Journal of asthma and allergy · 2019Review
- In Vitro Diagnosis of Immediate Drug Hypersensitivity Anno 2017: Potentials and Limitations.Drugs in R&D · 2017Review
- Pyrazolones metabolites are relevant for identifying selective anaphylaxis to metamizole.Scientific reports · 2016Article
- The Evolution of Human Basophil Biology from Neglect towards Understanding of Their Immune Functions.BioMed research international · 2016Review
- Asthma and Rhinitis Induced by Selective Immediate Reactions to Paracetamol and Non-steroidal Anti-inflammatory Drugs in Aspirin Tolerant Subjects.Frontiers in pharmacology · 2016Article
- Basophil Reactivity as Biomarker in Immediate Drug Hypersensitivity Reactions-Potential and Limitations.Frontiers in pharmacology · 2016Review
- Immediate-type hypersensitivity drug reactions.British journal of clinical pharmacology · 2014Review
- Recent applications of basophil activation tests in the diagnosis of drug hypersensitivity.Asia Pacific allergy · 2013Review
- Mediators released during human anaphylaxis.Current allergy and asthma reports · 2012Review
- Basophil activation test for investigation of IgE-mediated mechanisms in drug hypersensitivity.Journal of visualized experiments : JoVE · 2011Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
Abstract
backgroundHypersensitivity reactions against nonsteroidal antiinflammatory drugs (NSAIDs) like diclofenac (DF) can manifest as Type I-like allergic reactions including systemic anaphylaxis. However, except for isolated case studies experimental evidence for an IgE-mediated pathomechanism of DF hypersensitivity is lacking. In this study we aimed to investigate the possible involvement of drug- and/or metabolite-specific antibodies in selective DF hypersensitivity. METHODOLOGY/PRINCIPAL
findingsDF, an organochemically synthesized linkage variant, and five major Phase I metabolites were covalently coupled to carrier proteins. Drug conjugates were analyzed for coupling degree and capacity to crosslink receptor-bound IgE antibodies from drug-sensitized mice. With these conjugates, the presence of hapten-specific IgE antibodies was investigated in patients' samples by ELISA, mediator release assay, and basophil activation test. Production of sulfidoleukotrienes by drug conjugates was determined in PBMCs from DF-hypersensitive patients. All conjugates were shown to carry more than two haptens per carrier molecule. Immunization of mice with drug conjugates induced drug-specific IgE antibodies capable of triggering mediator release. Therefore, the conjugates are suitable tools for detection of drug-specific antibodies and for determination of their anaphylactic activity. Fifty-nine patients were enrolled and categorized as hypersensitive either selectively to DF or to multiple NSAIDs. In none of the patients' samples evidence for drug/metabolite-specific IgE in serum or bound to allergic effector cells was found. In contrast, a small group of patients (8/59, 14%) displayed drug/metabolite-specific IgG. CONCLUSIONS/SIGNIFICANCE: We found no evidence for an IgE-mediated effector mechanism based on haptenation of protein carriers in DF-hypersensitive patients. Furthermore, a potential involvement of the most relevant metabolites in DF hypersensitivity reactions could be excluded.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.