ArticlemBio2010
An engineered methanogenic pathway derived from the domains Bacteria and Archaea.
Article in mBio, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- An integrated culturomic and genomic database and analysis platform for methanogenic archaea.Database : the journal of biological databases and curation · 2026Article
- Teaching old dogs new tricks: genetic engineering methanogens.Applied and environmental microbiology · 2024Review
- A hybrid photocatalytic system enables direct glucose utilization for methanogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Model Organisms To Study Methanogenesis, a Uniquely Archaeal Metabolism.Journal of bacteriology · 2023Review
- Thorough Characterization ofFoods (Basel, Switzerland) · 2023Article
- CRISPR/Cas12a toolbox for genome editing inFrontiers in microbiology · 2023Article
- Insights into the biotechnology potential ofFrontiers in microbiology · 2022Review
- Anaerobic Production of Isoprene by EngineeredApplied and environmental microbiology · 2021Article
- Effects of low-dose contraceptive pills on the risk factors of cardiovascular diseases among 15-35-year-old women: A retrospective cohort.International journal of reproductive biomedicine · 2019Article
- Methanogens: pushing the boundaries of biology.Emerging topics in life sciences · 2018Article
- Methanogens: biochemical background and biotechnological applications.AMB Express · 2018Review
- Genome-Scale Metabolic Modeling of Archaea Lends Insight into Diversity of Metabolic Function.Archaea (Vancouver, B.C.) · 2017Review
- Expression of a bacterial catalase in a strictly anaerobic methanogen significantly increases tolerance to hydrogen peroxide but not oxygen.Microbiology (Reading, England) · 2014Article
- Genetic resources for methane production from biomass described with the Gene Ontology.Frontiers in microbiology · 2014Article
- Genome-scale metabolic reconstruction and hypothesis testing in the methanogenic archaeon Methanosarcina acetivorans C2A.Journal of bacteriology · 2012Article
- Genetic manipulation of Methanosarcina spp.Frontiers in microbiology · 2012Article
- Article
- Fundamentals of methanogenic pathways that are key to the biomethanation of complex biomass.Current opinion in biotechnology · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
A plasmid-based expression system wherein mekB was fused to a constitutive Methanosarcina acetivorans promoter was used to express MekB, a broad-specificity esterase from Pseudomonas veronii, in M. acetivorans. The engineered strain had 80-fold greater esterase activity than wild-type M. acetivorans. Methyl acetate and methyl propionate esters served as the sole carbon and energy sources, resulting in robust growth and methane formation, with consumption of >97% of the substrates. Methanol was undetectable at the end of growth with methyl acetate, whereas acetate accumulated, a result consistent with methanol as the more favorable substrate. Acetate was consumed, and growth continued after a period of adaptation. Similar results were obtained with methyl propionate, except propionate was not metabolized.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.