ArticlePloS one2010
Universal plant DNA barcode loci may not work in complex groups: a case study with Indian berberis species.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers, 2 of them syntheses that pooled it.
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Who cites it
69 citing papers in PubMed, 2 syntheses or guidelines pooled it, 191 citations in OpenAlex.
- Traditional System Versus DNA Barcoding in Identification of Bamboo Species: A Systematic Review.Molecular biotechnology · 2021Pooled it
- Utility of the trnH-psbA intergenic spacer region and its combinations as plant DNA barcodes: a meta-analysis.PloS one · 2012Pooled it
- Applying Target Capture Sequencing to Unravel thePlants (Basel, Switzerland) · 2026Article
- Limited resolution of DNA barcodes and environmental influence on phytochemical diversity in Berberis integerrima (Berberidaceae).Scientific reports · 2026Article
- Comparative analysis of monoterpene indole alkaloid composition and genotypic variation in ThaiFrontiers in plant science · 2026Article
- High species discrimination inPlant diversity · 2025Article
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- Characterization of the FourGenes · 2025Article
- Comparative plastome evaluation, phylogenomic analysis, and DNA signatures of medicinally important malvaceous genera and their adulterants.Scientific reports · 2025Article
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- COI Barcodes combined with multilocus data for representative Aporia taxa shed light on speciation in the high altitude Irano-Turanian mountain plateaus (Lepidoptera: Pieridae).BMC ecology and evolution · 2024Article
- Exploring DNA barcode for accurate identification of threatened Aconitum L. species from Western Himalaya.Molecular biology reports · 2024Article
- DNA barcode sequencing discovered the aecial host ofMycoscience · 2023Article
- DNA Barcoding of Potential Mosquito Disease Vectors (Diptera, Culicidae) in Jazan Region, Saudi Arabia.Pathogens (Basel, Switzerland) · 2022Article
- Quantification of adulteration in traded ayurvedic raw drugs employing machine learning approaches with DNA barcode database.3 Biotech · 2021Article
- Aromatic Profile Variation of Essential Oil from Dried Makwhaen Fruit and Related Species.Plants (Basel, Switzerland) · 2021Article
- Essential Oil Composition and DNA Barcode and Identification ofMolecules (Basel, Switzerland) · 2021Article
- Universal barcoding regions, rbcL, matK and trnH-psbA do not discriminate Cinnamomum species in Sri Lanka.PloS one · 2021Article
- Systematic reinstatement of highly sacredPhytoKeys · 2021Article
- Examining the utility of DNA barcodes for the identification of tallgrass prairie flora.Applications in plant sciences · 2021Article
9 more citing papers are in PubMed but not listed here.
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe concept of DNA barcoding for species identification has gained considerable momentum in animals because of fairly successful species identification using cytochrome oxidase I (COI). In plants, matK and rbcL have been proposed as standard barcodes. However, barcoding in complex genera is a challenging task. METHODOLOGY AND PRINCIPAL
findingsWe investigated the species discriminatory power of four reportedly most promising plant DNA barcoding loci (one from nuclear genome--ITS, and three from plastid genome--trnH-psbA, rbcL and matK) in species of Indian Berberis L. (Berberidaceae) and two other genera, Ficus L. (Moraceae) and Gossypium L. (Malvaceae). Berberis species were delineated using morphological characters. These characters resulted in a well resolved species tree. Applying both nucleotide distance and nucleotide character-based approaches, we found that none of the loci, either singly or in combinations, could discriminate the species of Berberis. ITS resolved all the tested species of Ficus and Gossypium and trnH-psbA resolved 82% of the tested species in Ficus. The highly regarded matK and rbcL could not resolve all the species. Finally, we employed amplified fragment length polymorphism test in species of Berberis to determine their relationships. Using ten primer pair combinations in AFLP, the data demonstrated incomplete species resolution. Further, AFLP analysis showed that there was a tendency of the Berberis accessions to cluster according to their geographic origin rather than species affiliation. CONCLUSIONS/SIGNIFICANCE: We reconfirm the earlier reports that the concept of universal barcode in plants may not work in a number of genera. Our results also suggest that the matK and rbcL, recommended as universal barcode loci for plants, may not work in all the genera of land plants. Morphological, geographical and molecular data analyses of Indian species of Berberis suggest probable reticulate evolution and thus barcode markers may not work in this case.
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