ArticleProceedings of the National Academy of Sciences of the United States of America2010
Tetrameric organization of vertebrate centromeric nucleosomes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
67 citing papers in PubMed, 109 citations in OpenAlex.
- Cryo-EM structures of CENP-A nucleosomes isolated from human cells.Communications biology · 2026Article
- CENP-A in cancer: from centromere identity to genome instability.Molecular biology reports · 2026Review
- The dynamic centromere.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Review
- High-resolution analysis of human centromeric chromatin.Life science alliance · 2025Article
- Native and tagged CENP-A histones are functionally inequivalent.Epigenetics & chromatin · 2024Article
- DNA satellite and chromatin organization at mouse centromeres and pericentromeres.Genome biology · 2024Article
- Article
- Molecular Complexes at Euchromatin, Heterochromatin and Centromeric Chromatin.International journal of molecular sciences · 2021Review
- FOXD1 regulates cell division in clear cell renal cell carcinoma.BMC cancer · 2021Article
- Nano-Surveillance: Tracking Individual Molecules in a Sea of Chromatin.Journal of molecular biology · 2021Review
- Stable inheritance of CENP-A chromatin: Inner strength versus dynamic control.The Journal of cell biology · 2020Article
- Review
- Intrinsic elasticity of nucleosomes is encoded by histone variants and calibrated by their binding partners.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Insights into Centromere DNA Bending Revealed by the Cryo-EM Structure of the Core Centromere Binding Factor 3 with Ndc10.Cell reports · 2018Article
- Human centromeric CENP-A chromatin is a homotypic, octameric nucleosome at all cell cycle points.The Journal of cell biology · 2017Article
- CENPT bridges adjacent CENPA nucleosomes on young human α-satellite dimers.Genome research · 2016Article
- Peering at Brain Polysomes with Atomic Force Microscopy.Journal of visualized experiments : JoVE · 2016Article
- Enhanced electrostatic force microscopy reveals higher-order DNA looping mediated by the telomeric protein TRF2.Scientific reports · 2016Article
- Recent insights from in vitro single-molecule studies into nucleosome structure and dynamics.Biophysical reviews · 2016Review
- Gene Expression and Chromatin Modifications Associated with Maize Centromeres.G3 (Bethesda, Md.) · 2015Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Mitosis ensures equal genome segregation in the eukaryotic lineage. This process is facilitated by microtubule attachment to each chromosome via its centromere. In centromeres, canonical histone H3 is replaced in nucleosomes by a centromere-specific histone H3 variant (CENH3), providing the unique epigenetic signature required for microtubule binding. Due to recent findings of alternative CENH3 nucleosomal forms in invertebrate centromeres, it has been debated whether the classical octameric nucleosomal arrangement of two copies of CENH3, H4, H2A, and H2B forms the basis of the vertebrate centromere. To address this question directly, we examined CENH3 [centromere protein A (CENP-A)] nucleosomal organization in human cells, using a combination of nucleosome component analysis, atomic force microscopy (AFM), and immunoelectron microscopy (immuno-EM). We report that native CENP-A nucleosomes contain centromeric alpha satellite DNA, have equimolar amounts of H2A, H2B, CENP-A, and H4, and bind kinetochore proteins. These nucleosomes, when measured by AFM, yield one-half the dimensions of canonical octameric nucleosomes. Using immuno-EM, we find that one copy of CENP-A, H2A, H2B, and H4 coexist in CENP-A nucleosomes, in which internal C-terminal domains are accessible. Our observations indicate that CENP-A nucleosomes are organized as asymmetric heterotypic tetramers, rather than canonical octamers. Such altered nucleosomes form a chromatin fiber with distinct folding characteristics, which we utilize to discriminate tetramers directly within bulk chromatin. We discuss implications of our observations in the context of universal epigenetic and mechanical requirements for functional centromeres.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.