ArticleDNA research : an international journal for rapid publication of reports on genes and genomes2010
Genopal™: a novel hollow fibre array for focused microarray analysis.
Article in DNA research : an international journal for rapid publication of reports on genes and genomes, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Consideration of Commercially Available Hepatocytes as Cell Sources for Liver-Microphysiological Systems by Comparing Liver Characteristics.Pharmaceutics · 2022Article
- Development of Oral Care Chip, a novel device for quantitative detection of the oral microbiota associated with periodontal disease.PloS one · 2020Article
- Identification of anti-inflammatory constituents in Phellodendri Cortex and Coptidis Rhizoma by monitoring the suppression of nitric oxide production.Journal of natural medicines · 2017Article
- Identification of potential gene targets in systemic vasculitis using DNA microarray analysis.Molecular medicine reports · 2017Article
- IFI27 Is a Useful Genetic Marker for Diagnosis of Immunoglobulin A Nephropathy and Membranous Nephropathy Using Peripheral Blood.PloS one · 2016Article
- Fluorescence-based bioassays for the detection and evaluation of food materials.Sensors (Basel, Switzerland) · 2015Review
- Development of a fibrous DNA chip for cost-effective β-thalassemia genotyping.International journal of hematology · 2012Article
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Authors and funding
8 authors.
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Abstract
Expression profiling of target genes in patient blood is a powerful tool for RNA diagnosis. Here, we describe Genopal™, a novel platform ideal for efficient focused microarray analysis. Genopal™, which consists of gel-filled fibres, is advantageous for high-quality mass production via large-scale slicing of the Genopal™ block. We prepared two arrays, infectant and autoimmunity, that provided highly reliable data in terms of repetitive scanning of the same and/or distinct microarrays. Moreover, we demonstrated that Genopal™ had sensitivity sufficient to yield signals in short hybridization times (0.5 h). Application of the autoimmunity array to blood samples allowed us to identify an expression pattern specific to Takayasu arteritis based on the Spearman rank correlation by comparing the reference profile with those of several autoimmune diseases and healthy volunteers (HVs). The comparison of these data with those obtained by other methods revealed that they exhibited similar expression profiles of many target genes. Taken together, these data demonstrate that Genopal™ is an advantageous platform for focused microarrays with regard to its low cost, rapid results and reliable quality.
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