ArticleReproductive biology and endocrinology : RB&E2010
Effects of luteinizing hormone-releasing hormone and arginine-vasotocin on the sperm-release response of Günther's Toadlet, Pseudophryne guentheri.
Article in Reproductive biology and endocrinology : RB&E, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 23 citations in OpenAlex.
- Recovery and Characterization of Spermatozoa in a Neotropical, Terrestrial, Direct-Developing Riparian Frog (Animals : an open access journal from MDPI · 2023Article
- Application of Reproductive Technologies to the Critically Endangered Baw Baw Frog,Animals : an open access journal from MDPI · 2023Article
- Amphibian reproductive technologies: approaches and welfare considerations.Conservation physiology · 2021Article
- The effect of injection and topical application of hCG and GnRH agonist to induce sperm-release in the roseate frog,Conservation physiology · 2020Article
- Hormone-induced sperm-release in the critically endangered Booroolong frog (Conservation physiology · 2019Article
- Effect of priming injections of luteinizing hormone-releasing hormone on spermiation and ovulation in Gϋnther's toadlet, Pseudophryne guentheri.Reproductive biology and endocrinology : RB&E · 2011Article
- Hormonal induction of gamete release, and in-vitro fertilisation, in the critically endangered southern corroboree frog, Pseudophryne corroboree.Reproductive biology and endocrinology : RB&E · 2010Article
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1 author at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundLuteinizing hormone-releasing hormone (LHRH) is an exogenous hormone commonly used to induce spermiation in anuran amphibians. Over the past few decades, the LHRH dose administered to individuals and the frequency of injection has been highly variable. The sperm-release responses reported have been correspondingly diverse, highlighting a need to quantify dose-response relationships on a species-specific basis. This study on the Australian anuran Pseudophryne guentheri first evaluated the spermiation response of males administered one of five LHRHa doses, and second, determined whether AVT administered in combination with the optimal LHRHa dose improved sperm-release.
methodsMale toadlets were administered a single dose of 0, 1, 2, 4 or 8 micrograms/g body weight of LHRHa. A 4 micrograms/g dose of AVT was administered alone or in combination with 2 micrograms/g LHRHa. Spermiation responses were evaluated at 3, 7 and 12 h post hormone administration (PA), and sperm number and viability were quantified using fluorescent microscopy.
resultsLHRHa administration was highly effective at inducing spermiation in P. guentheri, with 100% of hormone-treated males producing sperm during the experimental period. The number of sperm released in response to 2 micrograms/g LHRHa was greater than all other doses administered and sperm viability was highest in the 1 microgram/g treatment. The administration of AVT alone or in combination with LHRHa resulted in the release of significantly lower sperm numbers.
conclusionOverall, results from this study suggest that in P. guentheri, LHRHa is effective at inducing spermiation, but that AVT inhibits sperm-release.
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