ArticleMicrobial cell factories2010
Improved vanillin production in baker's yeast through in silico design.
Article in Microbial cell factories, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers.
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89 citing papers in PubMed, 272 citations in OpenAlex.
- Toward sustainable food preservatives: high-level production of sorbic acid in engineered Saccharomyces cerevisiae.Nature communications · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- SOD1 Deficiency Reveals Indirect Redox Stress Mechanisms Underlying Vanillin Toxicity inAntioxidants (Basel, Switzerland) · 2025Article
- Transforming a Historical Chemical Synthetic Route for Vanillin Starting from Renewable Eugenol to a Cell-Free Bi-Enzymatic Cascade.ChemSusChem · 2025Article
- Moving from genome-scale to community-scale metabolic models for the human gut microbiome.Nature microbiology · 2025Review
- Redesigned Pathway forJACS Au · 2025Article
- Semi-rational design and modification of phosphoketolase to improve the yield of tyrosol inSynthetic and systems biotechnology · 2025Article
- Genetic Engineering Approaches for the Microbial Production of Vanillin.Biomolecules · 2024Review
- OMICS and Other Advanced Technologies in Mycological Applications.Journal of fungi (Basel, Switzerland) · 2023Review
- A whiff of the future: functions of phenylalanine-derived aroma compounds and advances in their industrial production.Plant biotechnology journal · 2022Review
- Engineering Saccharomyces cerevisiae for production of the capsaicinoid nonivamide.Microbial cell factories · 2022Article
- Engineering artificial photosynthetic life-forms through endosymbiosis.Nature communications · 2022Article
- Biosynthesis of vanillin by different microorganisms: a review.World journal of microbiology & biotechnology · 2022Review
- Capture Hi-C reveals the influence on dynamic three-dimensional chromosome organization perturbed by genetic variation or vanillin stress inFrontiers in microbiology · 2022Article
- Multi-modular engineering of Saccharomyces cerevisiae for high-titre production of tyrosol and salidroside.Microbial biotechnology · 2021Article
- Microbial production of advanced biofuels.Nature reviews. Microbiology · 2021Review
- Histamine binding activity of surface-modified yeast by histamine binding protein (HBP).AMB Express · 2021Article
- Proteomic analysis revealed the roles of YRR1 deletion in enhancing the vanillin resistance of Saccharomyces cerevisiae.Microbial cell factories · 2021Article
- Model-guided development of an evolutionarily stable yeast chassis.Molecular systems biology · 2021Article
- Peroxisome compartmentalization of a toxic enzyme improves alkaloid production.Nature chemical biology · 2021Article
29 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundVanillin is one of the most widely used flavouring agents, originally obtained from cured seed pods of the vanilla orchid Vanilla planifolia. Currently vanillin is mostly produced via chemical synthesis. A de novo synthetic pathway for heterologous vanillin production from glucose has recently been implemented in baker's yeast, Saccharamyces cerevisiae. In this study we aimed at engineering this vanillin cell factory towards improved productivity and thereby at developing an attractive alternative to chemical synthesis.
resultsExpression of a glycosyltransferase from Arabidopsis thaliana in the vanillin producing S. cerevisiae strain served to decrease product toxicity. An in silico metabolic engineering strategy of this vanillin glucoside producing strain was designed using a set of stoichiometric modelling tools applied to the yeast genome-scale metabolic network. Two targets (PDC1 and GDH1) were selected for experimental verification resulting in four engineered strains. Three of the mutants showed up to 1.5 fold higher vanillin β-D-glucoside yield in batch mode, while continuous culture of the Δpdc1 mutant showed a 2-fold productivity improvement. This mutant presented a 5-fold improvement in free vanillin production compared to the previous work on de novo vanillin biosynthesis in baker's yeast.
conclusionUse of constraints corresponding to different physiological states was found to greatly influence the target predictions given minimization of metabolic adjustment (MOMA) as biological objective function. In vivo verification of the targets, selected based on their predicted metabolic adjustment, successfully led to overproducing strains. Overall, we propose and demonstrate a framework for in silico design and target selection for improving microbial cell factories.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.