ArticleEnvironmental microbiology2011
Engineering a novel c-di-GMP-binding protein for biofilm dispersal.
Article in Environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
44 citing papers in PubMed, 99 citations in OpenAlex.
- Biofilm-mediated antibiotic tolerance in bacterial pathogens: Integrated molecular networks and novel therapeutic avenues.Virulence · 2026Review
- Genomic Drivers of Biofilm Formation inMicroorganisms · 2025Article
- Genome-wide characterization of hypothiocyanite stress response inJournal of bacteriology · 2025Article
- Article
- Beyond Antibiotics: What the Future Holds.Antibiotics (Basel, Switzerland) · 2024Review
- Application of biofilm dispersion-based nanoparticles in cutting off reinfection.Applied microbiology and biotechnology · 2024Review
- Biofilm and wound healing: from bench to bedside.European journal of medical research · 2023Review
- Using next generation antimicrobials to target the mechanisms of infection.npj antimicrobials and resistance · 2023Review
- What makes another life possible in bacteria? Global regulators as architects of bacterial biofilms.World journal of microbiology & biotechnology · 2022Review
- Biofilms in Surgical Site Infections: Recent Advances and Novel Prevention and Eradication Strategies.Antibiotics (Basel, Switzerland) · 2022Review
- A diguanylate cyclase regulates biofilm formation in3 Biotech · 2022Article
- Mutations inInternational journal of molecular sciences · 2021Article
- Free-Living, Psychrotrophic Bacteria of the GenusmSystems · 2021Article
- Microbial biofilm ecology, in silico study of quorum sensing receptor-ligand interactions and biofilm mediated bioremediation.Archives of microbiology · 2021Review
- Targeting Biofilms Therapy: Current Research Strategies and Development Hurdles.Microorganisms · 2020Review
- Evaluation and improvement of phosphate solubilization by an isolated bacterium Pantoea agglomerans ZB.World journal of microbiology & biotechnology · 2020Article
- Gene Targets in Ocular PathogenicFrontiers in microbiology · 2019Article
- Regulating exopolysaccharide gene wcaF allows control of Escherichia coli biofilm formation.Scientific reports · 2018Article
- Engineered Living Materials: Prospects and Challenges for Using Biological Systems to Direct the Assembly of Smart Materials.Advanced materials (Deerfield Beach, Fla.) · 2018Review
- Functional Characterization of c-di-GMP Signaling-Related Genes in the ProbioticFrontiers in microbiology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Bacteria prefer to grow attached to themselves or an interface, and it is important for an array of applications to make biofilms disperse. Here we report simultaneously the discovery and protein engineering of BdcA (formerly YjgI) for biofilm dispersal using the universal signal 3,5-cyclic diguanylic acid (c-di-GMP). The bdcA deletion reduced biofilm dispersal, and production of BdcA increased biofilm dispersal to wild-type level. Since BdcA increases motility and extracellular DNA production while decreasing exopolysaccharide, cell length and aggregation, we reasoned that BdcA decreases the concentration of c-di-GMP, the intracellular messenger that controls cell motility through flagellar rotation and biofilm formation through synthesis of curli and cellulose. Consistently, c-di-GMP levels increase upon deleting bdcA, and purified BdcA binds c-di-GMP but does not act as a phosphodiesterase. Additionally, BdcR (formerly YjgJ) is a negative regulator of bdcA. To increase biofilm dispersal, we used protein engineering to evolve BdcA for greater c-di-GMP binding and found that the single amino acid change E50Q causes nearly complete removal of biofilms via dispersal without affecting initial biofilm formation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.