ReviewMethods in molecular biology (Clifton, N.J.)2011
In situ ligation: a decade and a half of experience.
Review in Methods in molecular biology (Clifton, N.J.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed.
- A Nonclinical Safety Evaluation of Cold Atmospheric Plasma for Medical Applications: The Role of Genotoxicity and Mutagenicity Studies.Life (Basel, Switzerland) · 2024Article
- Radiation-Induced DNA Damage in Uveal Melanoma Is Influenced by Dose Delivery and Chromosome 3 Status.Investigative ophthalmology & visual science · 2024Article
- Spatial mapping of the DNA adducts in cancer.DNA repair · 2023Review
- Sperm DNA integrity and male infertility: a narrative review and guide for the reproductive physicians.Translational andrology and urology · 2022Review
- TdT-dUTP DSB End Labeling (TUDEL), for Specific, Direct In Situ Labeling of DNA Double Strand Breaks.Methods in molecular biology (Clifton, N.J.) · 2022Article
- A slipped-CAG DNA-binding small molecule induces trinucleotide-repeat contractions in vivo.Nature genetics · 2020Article
- DNA damage measurements within tissue samples with Repair Assisted Damage Detection (RADD).Current research in biotechnology · 2019Article
- Early neuronal accumulation of DNA double strand breaks in Alzheimer's disease.Acta neuropathologica communications · 2019Article
- Targets for repair: detecting and quantifying DNA damage with fluorescence-based methodologies.Current opinion in biotechnology · 2019Review
- DNA single-strand break-induced DNA damage response causes heart failure.Nature communications · 2017Article
- Dual Detection of Nucleolytic and Proteolytic Markers of Lysosomal Cell Death: DNase II-Type Breaks and Cathepsin D.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Zebra Tail Amplification: Accelerated Detection of Apoptotic Blunt-Ended DNA Breaks by In Situ Ligation.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Quick Detection of DNase II-Type Breaks in Formalin-Fixed Tissue Sections.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Nanoblinker: Brownian motion powered bio-nanomachine for FRET detection of phagocytic phase of apoptosis.PloS one · 2014Article
- Fluorescent probes detecting the phagocytic phase of apoptosis: enzyme-substrate complexes of topoisomerase and DNA.Molecules (Basel, Switzerland) · 2011Article
- Short-term Effects of Non-invasive Physical Plasma Treatment on Genomic Stability.In vivo (Athens, Greece)Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
The in situ ligation (ISL) methodology detects apoptotic cells by the presence of characteristic DNA double-strand breaks. A labeled double-stranded probe is ligated to the double-strand breaks in situ on tissue sections. Like the popular TUNEL assay, ISL detects cells in apoptosis based on the ongoing destruction of DNA by apoptotic nucleases. In comparison to TUNEL, it is more specific for apoptosis versus other causes of DNA damage, both repairable damage and necrosis. In the decade and a half since its introduction, ISL has been used in several hundred publications. Here we review the development of the method, its current status, and its uses and limitations.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.