ArticleCytotechnology2011
Increasing batch-to-batch reproducibility of CHO cultures by robust open-loop control.
Article in Cytotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed.
- Unveiling the potential of specific growth rate control in fed-batch fermentation: bridging the gap between product quantity and quality.World journal of microbiology & biotechnology · 2024Review
- Mechanistic Mathematical Models as a Basis for Digital Twins.Advances in biochemical engineering/biotechnology · 2021Article
- Dielectric Spectroscopy and Optical Density Measurement for the Online Monitoring and Control of Recombinant Protein Production in Stably Transformed Drosophila melanogaster S2 Cells.Sensors (Basel, Switzerland) · 2018Article
- Model-Based Methods in the Biopharmaceutical Process Lifecycle.Pharmaceutical research · 2017Article
- Soft sensor for monitoring biomass subpopulations in mammalian cell culture processes.Biotechnology letters · 2017Article
- A control strategy to investigate the relationship between specific productivity and high-mannose glycoforms in CHO cells.Applied microbiology and biotechnology · 2016Article
- Increasing batch-to-batch reproducibility of CHO-cell cultures using a model predictive control approach.Cytotechnology · 2012Article
- A novel approach for using dielectric spectroscopy to predict viable cell volume (VCV) in early process development.Biotechnology progressArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In order to guarantee the quality of recombinant therapeutic proteins produced in mammalian cell systems, the straightforward approach in industry is to run the processes as reproducible as possible. It is first shown that considerable distortions in the currently operated processes appear when the initial cell density deviates from its nominal value. Small deviations in the initial cell mass may lead to severe deviations from the desired biomass trajectory. Next, it is shown how to design a fed-batch production process in such a way that it is robust with respect to variations in the viable cell density. A simple open loop strategy is proposed for that purpose. Here we show for the first time at animal cell cultures (CHO cells) that by means of an appropriate glutamine feed rate profile F(t), which keeps the specific growth rate of the cells on a predefined value below its maximal value while maintaining the viabilities on a high level, the diverging viable cell count profiles change over into a robust converging set of profiles. The CHO cells used to validate the procedure could be focused to any specific growth rates below μ(max).
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.