ArticleApplied and environmental microbiology2011
Effects of Mn and Fe levels on Bacillus subtilis spore resistance and effects of Mn2+, other divalent cations, orthophosphate, and dipicolinic acid on protein resistance to ionizing radiation.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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38 citing papers in PubMed, 84 citations in OpenAlex.
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- Surface display of a manganese-binding domain enhances production and stress resistance inFrontiers in microbiology · 2025Article
- The ternary complex of MnProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Excess manganese increases photosynthetic activity via enhanced reducing center and antenna plasticity in Chlorella vulgaris.Scientific reports · 2023Article
- Promoted Spore Formation ofIndian journal of microbiology · 2022Article
- Effects of Desiccation and Freezing on Microbial Ionizing Radiation Survivability: Considerations for Mars Sample Return.Astrobiology · 2022Article
- Multiscale X-ray study ofProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Experimental evolution of extremophile resistance to ionizing radiation.Trends in genetics : TIG · 2021Review
- What's new and notable in bacterial spore killing!World journal of microbiology & biotechnology · 2021Review
- Effect of Calcium and Manganese Supplementation on Heat Resistance of Spores ofFrontiers in microbiology · 2021Article
- Engineering Bacillus subtilis as a Versatile and Stable Platform for Production of Nanobodies.Applied and environmental microbiology · 2020Article
- Optimization of bacterial sporulation using economic nutrient for self-healing concrete.Journal of microbiology (Seoul, Korea) · 2020Article
- DNA Damage Kills Bacterial Spores and Cells Exposed to 222-Nanometer UV Radiation.Applied and environmental microbiology · 2020Article
- Improved Production of Spores and Bioactive Metabolites from Bacillus amyloliquefaciens in Solid-state Fermentation by a Rapid Optimization Process.Probiotics and antimicrobial proteins · 2019Article
- The Ser/Thr protein kinase PrkC imprints phenotypic memory inThe Journal of biological chemistry · 2019Article
- Membrane Proteomes and Ion Transporters inJournal of bacteriology · 2019Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spores of Bacillus subtilis strains with (wild type) or without (α(-)β(-)) most DNA-binding α/β-type small, acid-soluble proteins (SASP) were prepared in medium with additional MnCl(2) concentrations of 0.3 μM to 1 mM. These haploid spores had Mn levels that varied up to 180-fold and Mn/Fe ratios that varied up to 300-fold. However, the resistance of these spores to desiccation, wet heat, dry heat, and in particular ionizing radiation was unaffected by their level of Mn or their Mn/Fe ratio; this was also the case for wild-type spore resistance to hydrogen peroxide (H(2)O(2)). However, α(-)β(-) spores were more sensitive to H(2)O(2) when they had high Mn levels and a high Mn/Fe ratio. These results suggest that Mn levels alone are not essential for wild-type bacterial spores' extreme resistance properties, in particular ionizing radiation, although high Mn levels sensitize α(-)β(-) spores to H(2)O(2), probably by repressing expression of the auxiliary DNA-protective protein MrgA. Notably, Mn(2+) complexed with the abundant spore molecule dipicolinic acid (DPA) with or without inorganic phosphate was very effective at protecting a restriction enzyme against ionizing radiation in vitro, and Ca(2+) complexed with DPA and phosphate was also very effective in this regard. These latter data suggest that protein protection in spores against treatments such as ionizing radiation that generate reactive oxygen species may be due in part to the spores' high levels of DPA conjugated to divalent metal ions, predominantly Ca(2+), much like high levels of Mn(2+) complexed with small molecules protect the bacterium Deinococcus radiodurans against ionizing radiation.
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