ArticleBiochemical and biophysical research communications2010
Transcriptional regulation of the hypocretin/orexin gene by NR6A1.
Article in Biochemical and biophysical research communications, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 19 citations in OpenAlex.
- Genetic associations between orexin genes and phenotypes related to behavioral regulation in humans, including substance use.Molecular psychiatry · 2025Article
- Orexin Reserve: A Mechanistic Framework for the Role of Orexins (Hypocretins) in Addiction.Biological psychiatry · 2022Review
- The transcription factor HAND2 up-regulates transcription of theThe Journal of biological chemistry · 2020Article
- Involvement of PLAGL1/ZAC1 in hypocretin/orexin transcription.International journal of molecular medicine · 2019Article
- DEC2 modulates orexin expression and regulates sleep.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Modular organization of a hypocretin gene minimal promoter.Molecular medicine reports · 2018Article
- Reactivation of hyperglycemia-induced hypocretin (HCRT) gene silencing by N-acetyl-d-mannosamine in the orexin neurons derived from human iPS cells.Epigenetics · 2017Article
- Hypocretin neuron-specific transcriptome profiling identifies the sleep modulator Kcnh4a.eLife · 2015Article
- Genetic manipulation of the mouse developing hypothalamus through in utero electroporation.Journal of visualized experiments : JoVE · 2013Article
- Epigenetic switching by the metabolism-sensing factors in the generation of orexin neurons from mouse embryonic stem cells.The Journal of biological chemistry · 2013Article
- Circadian rhythms and sleep--the metabolic connection.Pflugers Archiv : European journal of physiology · 2012Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
The hypocretin (also known as orexin) neuropeptide system coordinates the regulation of various physiological processes. A reduction in Nr6a1 expression was observed in hypocretin neuron-ablated transgenic mice. To show that prepro-hypocretin transcription is functionally modulated by NR6A1, we performed chromatin immunoprecipitation (ChIP) analysis, double-immunostaining, a luciferase reporter assay, and an in utero electroporation study. ChIP analysis showed that endogenous NR6A1 binds to a putative NR6A1-binding site. Double-immunostaining indicated almost all hypocretin neurons were positive for NR6A1 immunoreactivity. NR6A1 overexpression in SH-SY5Y cells modulated hypocretin promoter activity, an effect that was countered by lacking a putative NR6A1-binding site. Electroporation with Nr6a1 in the foetal hypothalamus promoted hypocretin transcription as compared to GFP-electroporation. These experiments confirmed that NR6A1 works as a regulator for hypocretin transcription.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.