ArticleJournal of structural biology2011
Dual-axis electron tomography of biological specimens: Extending the limits of specimen thickness with bright-field STEM imaging.
Article in Journal of structural biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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Who cites it
25 citing papers in PubMed.
- Ultrastructure of astrocytes using volume electron microscopy: A scoping review.The Journal of physiology · 2026Article
- High-Pressure Freezing EM Tomography of Entire Ribbon Synapses in the Retina.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Application of STEM tomography to investigate smooth ER morphology under stress conditions.Journal of microscopy · 2025Article
- Estimating the glutamate transporter surface density in distinct sub-cellular compartments of mouse hippocampal astrocytes.PLoS computational biology · 2022Article
- Review
- Nanoscale chromatin imaging and analysis platform bridges 4D chromatin organization with molecular function.Science advances · 2021Article
- Circadian Modulation of Neurons and Astrocytes Controls Synaptic Plasticity in Hippocampal Area CA1.Cell reports · 2020Article
- HPS1 Regulates the Maturation of Large Dense Core Vesicles and Lysozyme Secretion in Paneth Cells.Frontiers in immunology · 2020Article
- Tomographic Collection of Block-Based Sparse STEM Images: Practical Implementation and Impact on the Quality of the 3D Reconstructed Volume.Materials (Basel, Switzerland) · 2019Article
- AuTom-dualx: a toolkit for fully automatic fiducial marker-based alignment of dual-axis tilt series with simultaneous reconstruction.Bioinformatics (Oxford, England) · 2019Article
- Comparison of 3D cellular imaging techniques based on scanned electron probes: Serial block face SEM vs. Axial bright-field STEM tomography.Journal of structural biology · 2018Article
- Electron ptychographic microscopy for three-dimensional imaging.Nature communications · 2017Article
- Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography.Journal of visualized experiments : JoVE · 2017Article
- PAR1 activation induces rapid changes in glutamate uptake and astrocyte morphology.Scientific reports · 2017Article
- The cellular basis of platelet secretion: Emerging structure/function relationships.Platelets · 2017Review
- Compressed Sensing Electron Tomography for Determining Biological Structure.Scientific reports · 2016Article
- STEM tomography reveals that the canalicular system and α-granules remain separate compartments during early secretion stages in blood platelets.Journal of thrombosis and haemostasis : JTH · 2016Article
- The Atg17-Atg31-Atg29 Complex Coordinates with Atg11 to Recruit the Vam7 SNARE and Mediate Autophagosome-Vacuole Fusion.Current biology : CB · 2016Review
- Article
- PSD-95 family MAGUKs are essential for anchoring AMPA and NMDA receptor complexes at the postsynaptic density.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The absence of imaging lenses after the specimen in the scanning transmission electron microscope (STEM) enables electron tomography to be performed in the STEM mode on micrometer-thick plastic-embedded specimens without the deleterious effect of chromatic aberration, which limits spatial resolution and signal-to-noise ratio in conventional TEM. Using Monte Carlo calculations to simulate electron scattering from gold nanoparticles situated at the top and bottom surfaces of a plastic section, we assess the optimal acquisition strategy for axial bright-field STEM electron tomography at a beam-energy of 300keV. Dual tilt-axis STEM tomography with optimized axial bight-field detector geometry is demonstrated by application to micrometer-thick sections of beta cells from mouse pancreatic islet. The quality of the resulting three-dimensional reconstructions is comparable to that obtained from much thinner (0.3-micrometer) sections using conventional TEM tomography. The increased range of specimen thickness accessible to axial STEM tomography without the need for serial sectioning enables the 3-D visualization of more complex and larger subcellular structures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.