ArticleProceedings of the National Academy of Sciences of the United States of America1990
Regulation of interleukin 3 mRNA expression in mast cells occurs at the posttranscriptional level and is mediated by calcium ions.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 161 citations in OpenAlex.
- Effect of Kisspeptin on the Developmental Competence and Early Transcript Expression in Porcine Oocytes Parthenogenetically Activated with Different Methods.BioMed research international · 2018Article
- WT1-associated protein is a novel prognostic factor in pancreatic ductal adenocarcinoma.Oncology letters · 2017Article
- Post-transcriptional regulatory networks in immunity.Immunological reviews · 2013Review
- Role of CSF-1 in progression of epithelial ovarian cancer.Future oncology (London, England) · 2009Review
- Regulation of Ca2+ signaling with particular focus on mast cells.Critical reviews in immunology · 2009Review
- Wilms' tumor 1-associating protein regulates G2/M transition through stabilization of cyclin A2 mRNA.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- RNA-binding protein HuR is required for stabilization of SLC11A1 mRNA and SLC11A1 protein expression.Molecular and cellular biology · 2005Article
- Neuronal ELAV proteins enhance mRNA stability by a PKCalpha-dependent pathway.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- The ARE-dependent mRNA-destabilizing activity of BRF1 is regulated by protein kinase B.The EMBO journal · 2004Article
- A GFP-based assay for rapid screening of compounds affecting ARE-dependent mRNA turnover.Nucleic acids research · 2004Article
- Regulation of nuclear gamma interferon gene expression by interleukin 12 (IL-12) and IL-2 represents a novel form of posttranscriptional control.Molecular and cellular biology · 2002Article
- Cellular mutants define a common mRNA degradation pathway targeting cytokine AU-rich elements.RNA (New York, N.Y.) · 2001Article
- Parallel and independent regulation of interleukin-3 mRNA turnover by phosphatidylinositol 3-kinase and p38 mitogen-activated protein kinase.Molecular and cellular biology · 2001Article
- Regulation of Nramp1 mRNA stability by oxidants and protein kinase C in RAW264.7 macrophages expressing Nramp1(Gly169).The Biochemical journal · 2000Article
- HuR regulates cyclin A and cyclin B1 mRNA stability during cell proliferation.The EMBO journal · 2000Article
- HuR regulates p21 mRNA stabilization by UV light.Molecular and cellular biology · 2000Article
- Evidence against a major role for Ca2+ in hypoxia-induced gene expression in human hepatoma cells (Hep3B).The Journal of physiology · 1999Article
- Article
- p21WAF1 expression by an activator of protein kinase C is regulated mainly at the post-transcriptional level in cells lacking p53: important role of RNA stabilization.The Biochemical journal · 1999Article
- Calcium-induced stabilization of AU-rich short-lived mRNAs is a common default response.Gene expression · 1999Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Interleukin 3 (IL-3) is transiently produced by murine bone marrow-derived mast cells in response to antigen stimulation of the high-affinity immunoglobulin E receptors. We have studied the postreceptor signaling pathways involved in regulating expression of the IL-3 gene in the murine mast cell line PB-3c. Large amounts of IL-3 mRNA accumulated after exposure of cells to calcium ionophore A23187, a reagent that increases intracellular Ca2+. Phorbol 12-myristate 13-acetate, which stimulates protein kinase C, did not induce IL-3 mRNA accumulation, although it did potentiate the effect of A23187. Nuclear run-on analysis showed that the IL-3 gene is constitutively transcribed in unstimulated cells and that treatment with A23187 and/or phorbol ester has no influence on its transcription rate. The effect of A23187 was found to be due to stabilization of the IL-3 mRNA. In cells maintained in the presence of A23187 the IL-3 mRNA was stable during 3 hr of incubation with actinomycin D, whereas removal of A23187 under the same conditions resulted in rapid degradation of the mRNA. These results indicate that control of expression of the IL-3 gene in mast cells is primarily at the posttranscriptional level and that the Ca2(+)-dependent signal-transduction pathway plays an important role in this process. Synthesis of granulocyte/macrophage colony-stimulating factor mRNA in response to A23187 and phorbol ester was found to be subject to both transcriptional and posttranscriptional regulation.
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