ArticleMolecular ecology2010
Genomic-scale capture and sequencing of endogenous DNA from feces.
Article in Molecular ecology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
56 citing papers in PubMed.
- Individual Identification of Prey in Carnivore Scats.Molecular ecology resources · 2026Article
- Maximizing the Quality of Non-Invasive Samples for Conservation Genetics Using Targeted Next-Generation Sequencing: A Comparison of Fecal DNA Preservation Methodologies.Ecology and evolution · 2026Article
- DNA purity over yield: optimizing extraction and PCR in degraded Asian elephant (Elephas maximus) fecal samples.Genes & genomics · 2026Article
- Article
- Assessment of High Throughput Sequencing Quality of Host DNA Enriched From Faeces: A Case From Captive Tiger.Molecular ecology resources · 2026Article
- Individual identification and kinship testing from hair shaft nuclear DNA: leveraging short amplicon strategy and bioinformatics models.International journal of legal medicine · 2026Article
- clusIBD: Robust Detection of Identity-by-descent Segments Using Unphased Genetic Data from Poor-quality Samples.Genomics, proteomics & bioinformatics · 2025Article
- Scaling-up RADseq methods for large datasets of non-invasive samples: Lessons for library construction and data preprocessing.Molecular ecology resources · 2025Article
- Age Is Just a Number: No Impacts of Scat Ageing on Single Nucleotide Polymorphism Genotyping Using a Target Capture Approach.Ecology and evolution · 2025Article
- Comparison of Fecal DNA Extraction Kits for the Giant Panda (Ecology and evolution · 2025Article
- Local genetic adaptation to habitat in wild chimpanzees.Science (New York, N.Y.) · 2025Article
- Genome-scale evolution in local populations of wild chimpanzees.Scientific reports · 2025Article
- FecalSeq enrichment with RAD Sequencing from non-invasive environmental samples holds promise for genetic monitoring of an imperiled lagomorph.Scientific reports · 2024Article
- Local genetic adaptation to habitat in wild chimpanzees.bioRxiv : the preprint server for biology · 2024Article
- Evaluating genotyping-in-thousands by sequencing as a genetic monitoring tool for a climate sentinel mammal using non-invasive and archival samples.Ecology and evolution · 2024Article
- Evaluating the use of non-invasive hair sampling and ddRAD to characterize populations of endangered species: Application to a peripheral population of the European mink.Ecology and evolution · 2023Article
- Genotyping-in-thousands by sequencing (GT-seq) of noninvasive faecal and degraded samples: A new panel to enable ongoing monitoring of Canadian polar bear populations.Molecular ecology resources · 2022Article
- Population dynamics and genetic connectivity in recent chimpanzee history.Cell genomics · 2022Article
- How methodological changes have influenced our understanding of population structure in threatened species: insights from tiger populations across India.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Combining methods for non-invasive fecal DNA enables whole genome and metagenomic analyses in wildlife biology.Frontiers in genetics · 2022Article
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Abstract
Genomic-level analyses of DNA from non-invasive sources would facilitate powerful conservation and evolutionary studies in natural populations of endangered and otherwise elusive species. However, the typical low quantity and poor quality of DNA that is extracted from non-invasive samples have generally precluded such work. Here we apply a modified DNA capture protocol that, when used in combination with massively-parallel sequencing technology, facilitates efficient and highly-accurate resequencing of megabases of specified nuclear genomic regions from fecal DNA samples. We validated our approach by comparing genetic variants identified from corresponding fecal and blood DNA samples of six western chimpanzees (Pan troglodytes verus) across more than 1.5 megabases of chromosome 21, chromosome X, and the complete mitochondrial genome. Our results suggest that it is now feasible to conduct genomic studies in natural populations for which constraints on invasive sampling have otherwise long been a barrier. The data we collected also provided an opportunity to examine western chimpanzee genetic diversity at unprecedented scale. Despite high mitochondrial genome diversity (π = 0.585%), western chimpanzees have a low ratio (0.42) of X chromosomal (π = 0.034%) to autosomal (chromosome 21 π = 0.081%) sequence diversity, a pattern that may reflect an unusual demographic history of this subspecies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.