Evidence map›Paper›PMID 21054079›Full record

ArticleJournal of biomedical optics

On the use of fluorescence probes for detecting reactive oxygen and nitrogen species associated with photodynamic therapy.

Michael Price, David Kessel

Abstract readEvaluation Study
In one paragraph

Article in Journal of biomedical optics. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Critical PDT Theory III: Events at the Molecular and Cellular Level.International journal of molecular sciences · 2022
    Review
  2. Article
  3. Article
  4. Article
  5. Photosensitization in Porphyrias and Photodynamic Therapy Involves TRPA1 and TRPV1.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016
    Article
  6. Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Michael PriceWayne State University School of Medicine, Cancer Biology Program, Detroit, Michigan, 48201, USA.
David Kessel

Funding

THE ROLE OF CNS INSULIN AS A MODULATOR OF DIABETIC COMPLICATIONSR25GM058905 · NIGMS · WAYNE STATE UNIVERSITY · PI CHOW, CHRISTINE S, DUNBAR, JOSEPH C · 1999 to 2020
$14.4M
Subcellular targets and PDT-induces cell deathR01CA023378 · NCI · WAYNE STATE UNIVERSITY · PI KESSEL, DAVID HARRY · 1985 to 2013
$3.3M
NCI NIH HHS CA 23378NCI NIH HHS R01 CA023378NIGMS NIH HHS GM058905-11NIGMS NIH HHS R25 GM058905
6 · The paper itself

Abstract

Fluorescent probes are frequently employed for the detection of different reactive oxygen and nitrogen species formed during the irradiation of photosensitized cells and tissues. Investigators often interpret the results in terms of information provided with the different probes without examining specificity or determinants of fluorogenic reactions. We examine five fluorescent probes in a cell-free system: reduced 2',7'-dichlorofluorescein, dihydroethidine, dihydrorhodamine, 3'-(p aminophenyl) fluorescein (APF), and 4',5'-diaminofluorescein. Of these, only APF demonstrates a high degree of specificity for a single reactive species. There is a substantial influence of peroxidase activity on all fluorogenic interactions. The fluorescence of the photosensitizing agent also must be taken into account in evaluating results.

Indexed as

Fluorescent DyesPhotochemotherapyAniline CompoundsAnimalsCell-Free SystemCell Line, TumorDicarbethoxydihydrocollidineFluoresceinsLeukemia P388MiceMicroscopy, FluorescenceMicroscopy, Phase-ContrastReactive Nitrogen SpeciesReactive Oxygen SpeciesRhodamines2',7'-dichlorofluoresceinaminophenyl fluoresceinAniline CompoundsDicarbethoxydihydrocollidinedihydroethidineFluoresceinsFluorescent DyesReactive Nitrogen SpeciesReactive Oxygen SpeciesRhodamines

Identifiers

PMID21054079
PMCPMC2945737

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.