ArticleCurrent protocols in microbiology2010
Genome-wide fitness and genetic interactions determined by Tn-seq, a high-throughput massively parallel sequencing method for microorganisms.
Article in Current protocols in microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed, 116 citations in OpenAlex.
- Determination of the chromosomal position effects for plug-and-play application in theSynthetic and systems biotechnology · 2024Article
- Temporal genome-wide fitness analysis ofmSystems · 2024Article
- Airway proteolytic control of pneumococcal competence.PLoS pathogens · 2023Article
- A genome-wide atlas of antibiotic susceptibility targets and pathways to tolerance.Nature communications · 2022Article
- Integrated genomics and chemical biology herald an era of sophisticated antibacterial discovery, from defining essential genes to target elucidation.Cell chemical biology · 2022Review
- Genome-Wide fitness analysis of group B Streptococcus in human amniotic fluid reveals a transcription factor that controls multiple virulence traits.PLoS pathogens · 2021Article
- Transposon Mutagenesis Screen of Klebsiella pneumoniae Identifies Multiple Genes Important for Resisting Antimicrobial Activities of Neutrophils in Mice.Infection and immunity · 2020Article
- Adaptation to Adversity: the Intermingling of Stress Tolerance and Pathogenesis in Enterococci.Microbiology and molecular biology reviews : MMBR · 2019Review
- Bacterial Factors Required for Transmission of Streptococcus pneumoniae in Mammalian Hosts.Cell host & microbe · 2019Article
- Survival of Anaerobic FeJournal of bacteriology · 2018Article
- Genome-Wide Screens Reveal New Gene Products That Influence Genetic Competence in Streptococcus mutans.Journal of bacteriology · 2018Article
- The Streptococcus agalactiae Stringent Response Enhances Virulence and Persistence in Human Blood.Infection and immunity · 2018Article
- Genome Scale Mutational Analysis of Geobacter sulfurreducens Reveals Distinct Molecular Mechanisms for Respiration and Sensing of Poised Electrodes versus Fe(III) Oxides.Journal of bacteriology · 2017Article
- Genome-Wide Survey of Pseudomonas aeruginosa PA14 Reveals a Role for the Glyoxylate Pathway and Extracellular Proteases in the Utilization of Mucin.Infection and immunity · 2017Article
- GacA is essential for Group A Streptococcus and defines a new class of monomeric dTDP-4-dehydrorhamnose reductases (RmlD).Molecular microbiology · 2015Article
- High-throughput bacterial functional genomics in the sequencing era.Current opinion in microbiology · 2015Review
- Tn-seq explorer: a tool for analysis of high-throughput sequencing data of transposon mutant libraries.PloS one · 2015Article
- Identification of host genes that affect acquisition of an integrative and conjugative element in Bacillus subtilis.Molecular microbiology · 2014Article
- Genomic analyses of pneumococci from children with sickle cell disease expose host-specific bacterial adaptations and deficits in current interventions.Cell host & microbe · 2014Article
- High-throughput profiling of influenza A virus hemagglutinin gene at single-nucleotide resolution.Scientific reports · 2014Article
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2 authors at 1 institution in 1 country.
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Abstract
The lagging annotation of bacterial genomes and the inherent genetic complexity of many phenotypes is hindering the discovery of new drug targets and the development of new antimicrobials and vaccines. Here we present the method Tn-seq, with which it has become possible to quantitatively determine fitness for most genes in a microorganism and to screen for quantitative genetic interactions on a genome-wide scale and in a high-throughput fashion. Tn-seq can thus direct studies in the annotation of genes and untangle complex phenotypes. The method is based on the construction of a saturated Mariner transposon insertion library. After library selection, changes in frequency of each insertion mutant are determined by sequencing of the flanking regions en masse. These changes are used to calculate each mutant's fitness. The method has been developed for the Gram-positive bacterium Streptococcus pneumoniae, a causative agent of pneumonia and meningitis; however, due to the wide activity of the Mariner transposon, Tn-seq can be applied to many different microbial species.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.