ArticleStem cell reviews and reports2011
Human stem cell cultures from cleft lip/palate patients show enrichment of transcripts involved in extracellular matrix modeling by comparison to controls.
Article in Stem cell reviews and reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 58 citations in OpenAlex.
- Craniomaxillofacial-Derived MSCs in Congenital Defect Reconstruction.Biomolecules · 2025Review
- About a Possible Impact of Endodontic Infections byInternational journal of molecular sciences · 2024Review
- E-cadherin variants associated with oral facial clefts trigger aberrant cell motility in a REG1A-dependent manner.Cell communication and signaling : CCS · 2024Article
- Neurological Disease Modeling Using Pluripotent and Multipotent Stem Cells: A Key Step towards Understanding and Treating Mucopolysaccharidoses.Biomedicines · 2023Review
- Whole-genome sequencing reveals de-novo mutations associated with nonsyndromic cleft lip/palate.Scientific reports · 2022Article
- Functional impact of multi-omic interactions in breast cancer subtypes.Frontiers in genetics · 2022Article
- Dentin dysplasia type I-A dental disease with genetic heterogeneity.Oral diseases · 2019Review
- Network-based identification of critical regulators as putative drivers of human cleft lip.BMC medical genomics · 2019Article
- Extracellular Matrix Composition and Remodeling: Current Perspectives on Secondary Palate Formation, Cleft Lip/Palate, and Palatal Reconstruction.Frontiers in cell and developmental biology · 2019Review
- Gastrointestinal Transcriptomic Response of Metabolic Vitamin B12 Pathways in Roux-en-Y Gastric Bypass.Clinical and translational gastroenterology · 2017Article
- Clinical Aspects associated with Syndromic forms of Orofacial Clefts in a Colombian population.Colombia medica (Cali, Colombia) · 2015Article
- Genome-wide two-marker linkage disequilibrium mapping of quantitative trait loci.BMC genetics · 2014Article
- Susceptibility to DNA damage as a molecular mechanism for non-syndromic cleft lip and palate.PloS one · 2013Article
- Evolutionary dynamism of the primate LRRC37 gene family.Genome research · 2013Article
- Expression and mutation analyses implicate ARHGAP29 as the etiologic gene for the cleft lip with or without cleft palate locus identified by genome-wide association on chromosome 1p22.Birth defects research. Part A, Clinical and molecular teratology · 2012Article
- Association of MMP3 and TIMP2 promoter polymorphisms with nonsyndromic oral clefts.Birth defects research. Part A, Clinical and molecular teratology · 2012Article
- Genetics and management of the patient with orofacial cleft.Plastic surgery international · 2012Article
- Stem cell therapy: A novel treatment approach for oral mucosal lesions.Journal of pharmacy & bioallied sciencesReview
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nonsyndromic cleft lip and palate (NSCL/P) is a complex disease resulting from failure of fusion of facial primordia, a complex developmental process that includes the epithelial-mesenchymal transition (EMT). Detection of differential gene transcription between NSCL/P patients and control individuals offers an interesting alternative for investigating pathways involved in disease manifestation. Here we compared the transcriptome of 6 dental pulp stem cell (DPSC) cultures from NSCL/P patients and 6 controls. Eighty-seven differentially expressed genes (DEGs) were identified. The most significant putative gene network comprised 13 out of 87 DEGs of which 8 encode extracellular proteins: ACAN, COL4A1, COL4A2, GDF15, IGF2, MMP1, MMP3 and PDGFa. Through clustering analyses we also observed that MMP3, ACAN, COL4A1 and COL4A2 exhibit co-regulated expression. Interestingly, it is known that MMP3 cleavages a wide range of extracellular proteins, including the collagens IV, V, IX, X, proteoglycans, fibronectin and laminin. It is also capable of activating other MMPs. Moreover, MMP3 had previously been associated with NSCL/P. The same general pattern was observed in a further sample, confirming involvement of synchronized gene expression patterns which differed between NSCL/P patients and controls. These results show the robustness of our methodology for the detection of differentially expressed genes using the RankProd method. In conclusion, DPSCs from NSCL/P patients exhibit gene expression signatures involving genes associated with mechanisms of extracellular matrix modeling and palate EMT processes which differ from those observed in controls. This comparative approach should lead to a more rapid identification of gene networks predisposing to this complex malformation syndrome than conventional gene mapping technologies.
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