ArticleInvestigative ophthalmology & visual science2011
Differential expression of neuronal genes in Müller glia in two- and three-dimensional cultures.
Article in Investigative ophthalmology & visual science, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 29 citations in OpenAlex.
- Retinal Inflammation and Reactive Müller Cells: Neurotrophins' Release and Neuroprotective Strategies.Biology · 2024Review
- The mechanics of the retina: Müller glia role on retinal extracellular matrix and modelling.Frontiers in medicine · 2024Review
- Engineering a multicompartment in vitro model for dorsal root ganglia phenotypic assessment.Journal of biomedical materials research. Part B, Applied biomaterials · 2023Article
- Harnessing the Neuroprotective Behaviors of Müller Glia for Retinal Repair.Frontiers in bioscience (Landmark edition) · 2022Review
- Derivation and Characterization of Murine and Amphibian Müller Glia Cell Lines.Translational vision science & technology · 2022Article
- VEGF Upregulates EGFR Expression to Stimulate Chemotactic Behaviors in the rMC-1 Model of Müller Glia.Brain sciences · 2020Article
- Stimulation of Retinal Pigment Epithelium With an α7 nAChR Agonist Leads to Müller Glia Dependent Neurogenesis in the Adult Mammalian Retina.Investigative ophthalmology & visual science · 2019Article
- A low-cost microwell device for high-resolution imaging of neurite outgrowth in 3D.Journal of neural engineering · 2018Article
- Regulation of Stem Cell Properties of Müller Glia by JAK/STAT and MAPK Signaling in the Mammalian Retina.Stem cells international · 2017Review
- Nr2e1 regulates retinal lamination and the development of Müller glia, S-cones, and glycineric amacrine cells during retinogenesis.Molecular brain · 2015Article
- Article
- Distinct neurogenic potential in the retinal margin and the pars plana of mammalian eye.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Defining the integration capacity of embryonic stem cell-derived photoreceptor precursors.Stem cells (Dayton, Ohio) · 2012Article
- The rod photoreceptor lineage of teleost fish.Progress in retinal and eye research · 2011Review
- Eyes on DNA methylation: current evidence for DNA methylation in ocular development and disease.Journal of ocular biology, diseases, and informatics · 2011Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
purposeMüller glia in the mammalian retina have some stem cell-like characteristics, although their capacity for neurogenesis remains limited both in vivo and in vitro. In vitro studies to date have used traditional two-dimensional (2D) cell culture to assess neuronal differentiation of Müller glia. The purpose of this study was to compare the effects of 2D and three-dimensional (3D) environments on Müller glial gene expression after growth factor stimulation.
methodsConditionally immortalized mouse Müller glia cells (ImM10) were cultured under nonimmortalizing conditions with EGF/FGF2 to generate spheres that were differentiated in vitro on uncoated culture dishes (2D) or encapsulated in self-assembling, RADA-16 peptide hydrogels (3D) under identical media and growth factor supplementation conditions. Gene expression was analyzed using quantitative RT-PCR and immunocytochemistry. Cellular morphology was analyzed with light and confocal microscopy; sphere ultrastructure was analyzed with transmission electron microscopy.
resultsImM10 Müller cells express numerous genes associated with neural stem cells and retinal progenitors in both normal growth conditions and sphere-forming conditions. When encapsulated in the 3D hydrogel, cells can migrate and send processes into the hydrogel. Many genes associated with neurogenesis, as well as retinal neuron-specific genes, are differentially expressed in 2D and 3D differentiation conditions.
conclusionsImM10 Müller glia upregulate genes characteristic of retinal neurons after growth factor stimulation in vitro, and gene expression patterns are altered in 3D hydrogel cultures.
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