ArticleJournal of molecular biology2011
Temporal regulation of gene expression of the Thermus thermophilus bacteriophage P23-45.
Article in Journal of molecular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 36 citations in OpenAlex.
- Profiling the interplay and coevolution ofMicrobiology spectrum · 2024Article
- Tail-tape-fused virion and non-virion RNA polymerases of a thermophilic virus with an extremely long tail.Nature communications · 2024Article
- Engineering bacteriophages for enhanced host range and efficacy: insights from bacteriophage-bacteria interactions.Frontiers in microbiology · 2023Review
- Expression of a Phage-Encoded Gp21 Protein Protects Pseudomonas aeruginosa against Phage Infection.Journal of virology · 2022Article
- Phage-Encoded Endolysins.Antibiotics (Basel, Switzerland) · 2021Review
- Multisubunit RNA Polymerases of Jumbo Bacteriophages.Viruses · 2020Review
- Rethinking Phage Ecology by Rooting it Within an Established Plant Framework.PHAGE (New Rochelle, N.Y.) · 2020Article
- The replication machinery of LUCA: common origin of DNA replication and transcription.BMC biology · 2020Article
- The σ Subunit-Remodeling Factors: An Emerging Paradigms of Transcription Regulation.Frontiers in microbiology · 2020Review
- Structural basis for transcription antitermination at bacterial intrinsic terminator.Nature communications · 2019Article
- Biogeography and taxonomic overview of terrestrial hot spring thermophilic phages.Extremophiles : life under extreme conditions · 2018Review
- A Thermus phage protein inhibits host RNA polymerase by preventing template DNA strand loading during open promoter complex formation.Nucleic acids research · 2018Article
- Characterization of two related Erwinia myoviruses that are distant relatives of the PhiKZ-like Jumbo phages.PloS one · 2018Article
- Discovery of an expansive bacteriophage family that includes the most abundant viruses from the human gut.Nature microbiology · 2018Article
- A non-canonical multisubunit RNA polymerase encoded by the AR9 phage recognizes the template strand of its uracil-containing promoters.Nucleic acids research · 2017Article
- Transcription Profiling ofmBio · 2017Article
- RNA-Sequencing Reveals the Progression of Phage-Host Interactions between φR1-37 and Yersinia enterocolitica.Viruses · 2016Article
- Characterization of Five Novel Brevibacillus Bacteriophages and Genomic Comparison of Brevibacillus Phages.PloS one · 2016Article
- Article
- Complete Genome Sequence of Thermus aquaticus Y51MC23.PloS one · 2015Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 3 countries.
Funding
Abstract
Regulation of gene expression during infection of the thermophilic bacterium Thermus thermophilus HB8 with the bacteriophage P23-45 was investigated. Macroarray analysis revealed host transcription shut-off and identified three temporal classes of phage genes; early, middle and late. Primer extension experiments revealed that the 5' ends of P23-45 early transcripts are preceded by a common sequence motif that likely defines early viral promoters. T. thermophilus HB8 RNA polymerase (RNAP) recognizes middle and late phage promoters in vitro but does not recognize early promoters. In vivo experiments revealed the presence of rifampicin-resistant RNA polymerizing activity in infected cells responsible for early transcription. The product of the P23-45 early gene 64 shows a distant sequence similarity with the largest, catalytic subunits of multisubunit RNAPs and contains the conserved metal-binding motif that is diagnostic of these proteins. We hypothesize that ORF64 encodes rifampicin-resistant phage RNAP that recognizes early phage promoters. Affinity isolation of T. thermophilus HB8 RNAP from P23-45-infected cells identified two phage-encoded proteins, gp39 and gp76, that bind the host RNAP and inhibit in vitro transcription from host promoters, but not from middle or late phage promoters, and may thus control the shift from host to viral gene expression during infection. To our knowledge, gp39 and gp76 are the first characterized bacterial RNAP-binding proteins encoded by a thermophilic phage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.