ArticleReproductive biology and endocrinology : RB&E2010
Expression of genes related to the hypothalamic-pituitary-adrenal axis in murine fetal lungs in late gestation.
Article in Reproductive biology and endocrinology : RB&E, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 16 citations in OpenAlex.
- Leukemia Inhibitory Factor Induces Proopiomelanocortin via CRH/CRHR Pathway in Mouse Trophoblast.Frontiers in cell and developmental biology · 2021Article
- Prospective isolation of NKX2-1-expressing human lung progenitors derived from pluripotent stem cells.The Journal of clinical investigation · 2017Article
- Maternal immune activation epigenetically regulates hippocampal serotonin transporter levels.Neurobiology of stress · 2016Article
- Structural and transcriptomic response to antenatal corticosteroids in an Erk3-null mouse model of respiratory distress.American journal of obstetrics and gynecology · 2016Article
- Article
- A novel GABA-mediated corticotropin-releasing hormone secretory mechanism in the median eminence.Science advances · 2016Article
- Ontogeny of adrenal-like glucocorticoid synthesis pathway and of 20α-hydroxysteroid dehydrogenase in the mouse lung.BMC research notes · 2014Article
- Long-term effects of maternal immune activation on depression-like behavior in the mouse.Translational psychiatry · 2014Article
- Nicotine induced CpG methylation of Pax6 binding motif in StAR promoter reduces the gene expression and cortisol production.Toxicology and applied pharmacology · 2011Article
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4 authors at 1 institution in 1 country.
Funding
Abstract
backgroundLung maturation is modulated by several factors, including glucocorticoids. Expression of hypothalamic-pituitary-adrenal (HPA) axis-related components, with proposed or described local regulatory systems analogous to the HPA axis, was reported in peripheral tissues. Here, HPA axis-related genes were studied in the mouse developing lung during a period overlapping the surge of surfactant production.
methodsExpression of genes encoding for corticotropin-releasing hormone (CRH), CRH receptors (CRHR) 1 and 2beta, CRH-binding protein, proopiomelanocortin (POMC), melanocortin receptor 2 (MC2R), and glucocorticoid receptor was quantified by real-time PCR and localized by in situ hydridization in fetal lungs at gestational days (GD) 15.5, 16.5, and 17.5, and was also quantified in primary mesenchymal- and epithelial cell-enriched cultures. In addition, the capability of CRH and adrenocorticotropic hormone (ACTH) to stimulate pulmonary expression of enzymes involved in the adrenal pathway of glucocorticoid synthesis was addressed, as well as the glucocorticoid production by fetal lung explants.
resultsWe report that all the studied genes are expressed in fetal lungs according to different patterns. On GD 15.5, Mc2r showed peaks in expression in samples that have previously presented high mRNA levels for glucocorticoid synthesizing enzymes, including 11beta-hydroxylase (Cyp11b1). Crhr1 mRNA co-localized with Pomc mRNA in cells surrounding the proximal epithelium on GD 15.5 and 16.5. A transition in expression sites toward distal epithelial cells was observed between GD 15.5 and 17.5 for all the studied genes. CRH or ACTH stimulation of genes involved in the adrenal pathway of glucocorticoid synthesis was not observed in lung explants on GD 15.5, whereas CRH significantly increased expression of 21-hydroxylase (Cyp21a1) on GD 17.5. A deoxycorticosterone production by fetal lung explants was observed.
conclusionsTemporal and spatial modulations of expression of HPA axis-related genes in late gestation are consistent with roles for these genes in lung development. Our data are likely to lead to valuable insights in relation to lung diseases originating from lung immaturity.
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