ArticleBMC genomics2010
Integrative mapping analysis of chicken microchromosome 16 organization.
Article in BMC genomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Description of the complete rDNA repeat unit structure ofComparative cytogenetics · 2024Article
- A chromosome-level genome assembly for the Silkie chicken resolves complete sequences for key chicken metabolic, reproductive, and immunity genes.Communications biology · 2023Article
- Evolutionary analysis of a complete chicken genome.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
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- Evolution and molecular interactions of major histocompatibility complex (MHC)-G, -E and -F genes.Cellular and molecular life sciences : CMLS · 2022Review
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- Fourth Report on Chicken Genes and Chromosomes 2022.Cytogenetic and genome research · 2022Review
- Why Do Some Vertebrates Have Microchromosomes?Cells · 2021Review
- Review
- A simple means for MHC-Y genotyping in chickens using short tandem repeat sequences.Immunogenetics · 2020Article
- Structure of the intergenic spacers in chicken ribosomal DNA.Genetics, selection, evolution : GSE · 2019Article
- Avian Expression Patterns and Genomic Mapping Implicate Leptin in Digestion and TNF in Immunity, Suggesting That Their Interacting Adipokine Role Has Been Acquired Only in MammalsInternational journal of molecular sciences · 2019Article
- Genetic Mutation Analysis of High and Low IgY Chickens by Capture Sequencing.Animals : an open access journal from MDPI · 2019Article
- Review
- Genetic features of red and green junglefowls and relationship with Indonesian native chickens Sumatera and Kedu Hitam.BMC genomics · 2016Article
- Brief review of the chicken Major Histocompatibility Complex: the genes, their distribution on chromosome 16, and their contributions to disease resistance.Poultry science · 2016Review
- Signatures of Crested Ibis MHC Revealed by Recombination Screening and Short-Reads Assembly Strategy.PloS one · 2016Article
- Chicken rRNA Gene Cluster Structure.PloS one · 2016Article
- Exact approaches for scaffolding.BMC bioinformatics · 2015Article
- Conservation and losses of non-coding RNAs in avian genomes.PloS one · 2015Article
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe chicken karyotype is composed of 39 chromosome pairs, of which 9 still remain totally absent from the current genome sequence assembly, despite international efforts towards complete coverage. Some others are only very partially sequenced, amongst which microchromosome 16 (GGA16), particularly under-represented, with only 433 kb assembled for a full estimated size of 9 to 11 Mb. Besides the obvious need of full genome coverage with genetic markers for QTL (Quantitative Trait Loci) mapping and major genes identification studies, there is a major interest in the detailed study of this chromosome because it carries the two genetically independent MHC complexes B and Y. In addition, GGA16 carries the ribosomal RNA (rRNA) genes cluster, also known as the NOR (nucleolus organizer region). The purpose of the present study is to construct and present high resolution integrated maps of GGA16 to refine its organization and improve its coverage with genetic markers.
resultsWe developed 79 STS (Sequence Tagged Site) markers to build a physical RH (radiation hybrid) map and 34 genetic markers to extend the genetic map of GGA16. We screened a BAC (Bacterial Artificial Chromosome) library with markers for the MHC-B, MHC-Y and rRNA complexes. Selected clones were used to perform high resolution FISH (Fluorescent In Situ Hybridization) mapping on giant meiotic lampbrush chromosomes, allowing meiotic mapping in addition to the confirmation of the order of the three clusters along the chromosome. A region with high recombination rates and containing PO41 repeated elements separates the two MHC complexes.
conclusionsThe three complementary mapping strategies used refine greatly our knowledge of chicken microchromosome 16 organisation. The characterisation of the recombination hotspots separating the two MHC complexes demonstrates the presence of PO41 repetitive sequences both in tandem and inverted orientation. However, this region still needs to be studied in more detail.
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