ReviewAssay and drug development technologies2011
Cell-based screening using high-throughput flow cytometry.
Review in Assay and drug development technologies, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The role of alemtuzumab in the development of secondary autoimmunity in multiple Sclerosis: a systematic review.Journal of neuroinflammation · 2024Pooled it
- Deconer: An Evaluation Toolkit for Reference-based Deconvolution Methods Using Gene Expression Data.Genomics, proteomics & bioinformatics · 2025Article
- Characterizing Extracellular Vesicles from Biological Fluids.Journal of visualized experiments : JoVE · 2025Article
- Imaging Flow Cytometry in HIV Infection Research: Advantages and Opportunities.Methods and protocols · 2025Review
- Isolation and characterization of Saccharomyces cerevisiae mutants with increased cell wall chitin using fluorescence-activated cell sorting.FEMS yeast research · 2024Article
- A low-cost, label-free microfluidic scanning flow cytometer for high-accuracy quantification of size and refractive index of particles.Lab on a chip · 2023Article
- The In-Cell Western immunofluorescence assay to monitor PROTAC mediated protein degradation.Methods in enzymology · 2023Article
- AliGater: a framework for the development of bioinformatic pipelines for large-scale, high-dimensional cytometry data.Bioinformatics advances · 2023Article
- Article
- High-throughput screening strategies for space-based radiation countermeasure discovery.Life sciences in space research · 2022Review
- Adjuvant Discovery via a High Throughput Screen using Human Primary Mononuclear Cells.bioRxiv : the preprint server for biology · 2022Article
- A flow cytometry-based neutralization assay for simultaneous evaluation of blocking antibodies against SARS-CoV-2 variants.Frontiers in immunology · 2022Article
- Hyperspectral multiphoton microscopy forOptica · 2020Article
- The Nephroprotective Effect of Lycopene on Renal Ischemic Reperfusion Injury: A Mouse Model.Indian journal of clinical biochemistry : IJCB · 2020Article
- A "No-Touch" Antibody-Staining Method of Adherent Cells for High-Throughput Flow Cytometry in 384-Well Microplate Format for Cell-Based Drug Library Screening.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2020Article
- The promise of single-cell mechanophenotyping for clinical applications.Biomicrofluidics · 2020Article
- Very rapid cloning, expression and identifying specificity of T-cell receptors for T-cell engineering.PloS one · 2020Article
- A Fully Automated High-Throughput Flow Cytometry Screening System Enabling Phenotypic Drug Discovery.SLAS discovery : advancing life sciences R & D · 2018Article
- Synthetic 10FN3-based mono- and bivalent inhibitors of MDM2/X function.Protein engineering, design & selection : PEDS · 2018Article
- Highly multiplexed and quantitative cell-surface protein profiling using genetically barcoded antibodies.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This review describes the use of high-throughput flow cytometry for performing multiplexed cell-based and bead-based screens. With the many advances in cell-based analysis and screening, flow cytometry has historically been underutilized as a screening tool largely due to the limitations in handling large numbers of samples. However, there has been a resurgence in the use of flow cytometry due to a combination of innovations around instrumentation and a growing need for cell-based and bead-based applications. The HTFC™ Screening System (IntelliCyt Corporation, Albuquerque, NM) is a novel flow cytometry-based screening platform that incorporates a fast sample-loading technology, HyperCyt®, with a two-laser, six-parameter flow cytometer and powerful data analysis capabilities. The system is capable of running multiplexed screening assays at speeds of up to 40 wells per minute, enabling the processing of a 96- and 384-well plates in as little as 3 and 12 min, respectively. Embedded in the system is HyperView®, a data analysis software package that allows rapid identification of hits from multiplexed high-throughput flow cytometry screening campaigns. In addition, the software is incorporated into a server-based data management platform that enables seamless data accessibility and collaboration across multiple sites. High-throughput flow cytometry using the HyperCyt technology has been applied to numerous assay areas and screening campaigns, including efflux transporters, whole cell and receptor binding assays, functional G-protein-coupled receptor screening, in vitro toxicology, and antibody screening.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.